银杏叶提取物抗鼠肾缺血再灌注损伤及对P选择素表达的影响  

Effects of Ginkgo biloba extract on anti-ischemia reperfusion injury and P-Selectin expression in rats kidney

作　　者：张万帆[1] 杨吉乡[2] 谢守霞[3] 原文鹏[3] 庞春平[4] 张欣洲[1] 

机构地区：[1]暨南大学医学院附属第二医院,深圳市人民医院肾内科 [2]暨南大学医学院附属第二医院,深圳市人民医院急诊科 [3]暨南大学医学院附属第二医院,深圳市人民医院药学部 [4]暨南大学医学院附属第二医院,深圳市人民医院病理科

出　　处：《中国临床药理学与治疗学》2004年第7期785-787,共3页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：深圳市科技局基金资助项目 (№ 19990 40 60 )

摘　　要：目的 :探讨银杏叶提取物保护大鼠肾脏缺血再灌注损伤作用与机理。方法 :SD大鼠随机分为假手术组、缺血再灌注组、银杏叶提取物组。通过HE染色观察肾组织损伤和中性粒细胞浸润 ,免疫组织化学方法观察P选择素的表达情况。结果 :与假手术组相比 ,缺血再灌注组肾小管上皮细胞呈明显的缺血性改变 ,P选择素表达明显增强 (P <0 .0 1) ,肾小球内中性粒细胞增加明显 (P <0 .0 0 1) ;银杏叶提取物组比缺血再灌注组肾小管上皮细胞缺血性改变减轻 ,P选择素表达减少 (P <0 .0 1) ,肾小球内中性粒细胞较缺血再灌注组减少 (P <0 .0 0 1)。结论 :银杏叶提取物减轻大鼠肾缺血再灌注损伤 。AIM: To investigate the effects of Ginkgo biloba extract on rats with renal ischemia reperfusion injury and its protective mechanism was discussed. METHODS: A model of renal ischemia reperfusion injury was set up. 34 SD rats were randomly divided into sham operated group (n=10), ischemia reperfusion group (n=12), and Ginkgo biloba extract injection group (n=12). The renal ischemic injury and neutrphil infiltrate in tissue stained with hematoxylin and eosin were observed. The expression of P-Selectin in kidney was evaluated by immunohistochemistry. RESULTS: As compared with sham operated group, some ischemic changes increased significantly in the renal tubular epithelial cells, P-selectin expression (P< 0.01) and neutrophils in glomerular increased in ischemia reperfusion group (P< 0.001). As compared with ischemia reperfusion group, some mild ischemic changes alleviated significantly in the renal tubular epithelial cells and P-selectin expression (P< 0.01) and neutrophils in glomerular decreased in Ginkgo biloba extract injection group (P< 0.001). CONCLUSION: The administration of Ginkgo biloba extract can alleviate rat renal ischemia reperfusion injury, and decrease P-selectin expression and neutrophils infiltration in glomerular.

关 键 词：银杏叶提取物 P选择素 缺血再灌注损伤 肾脏 中性粒细胞 

分 类 号：R285.5[医药卫生—中药学]