人参果茎尖脱毒培养与快速繁殖  

Virus-free culture of shoot tips and rapid propagation of Solanum muricatum

作　　者：翟进升[1] 邹爱兰[1] 常兴亚[1] 

机构地区：[1]阜阳师范学院生物系,安徽阜阳236032

出　　处：《植物资源与环境学报》2004年第3期41-43,共3页Journal of Plant Resources and Environment

基　　金：安徽省教育厅重点资助项目(99JL180Z)

摘　　要：　对人参果(SolanummuricatumAit)茎尖进行离体培养及快速繁殖。结果表明:适于外植体生长分化的培养基是不加任何激素或只加0.2mg·L-1NAA的MS培养基;用MS+0.5mg·L-1NAA培养基能促进组培苗茎尖快速伸长,有利于茎尖的剥离和脱毒培养;适于茎尖愈伤组织形成和分化的培养基为MS+0.2mg·L-1NAA+2.0mg·L-16 BA;适于壮苗快繁的培养基为MS+0.5mg·L-1NAA+0.1mg·L-1PP333。生物学和电镜检测结果表明,利用0.2～0.3mm茎尖培养的人参果试管苗已脱除病毒。The culture and rapid propagation of shoot tips of Solanum muricatum Ait were investigated. The results showed that MS medium with both no phytohormone or 0.2 mg·L^(-1) NAA was suitable to grow for explants. MS medium with 0.5 mg·L^(-1) NAA was suitable for rapidly lengthening of shoot tips and to isolate and culture of the shoot tips. The better medium for inducing and differentiating of callus was MS + 0.2mg·L^(-1) NAA + 2.0 mg·L^(-1) 6-BA, and the suitable medium for growth and rapid propagation was MS+0.5 mg·L^(-1) NAA+0.1 mg·L^(-1) PP_(333). The results of examination by the methods of biology and electron microscope showed that there were no viruses in the plantlets cultured from the shoot tips of 0.2-0.3 mm.

关 键 词：人参果 茎尖培养 脱毒培养 快速繁殖 培养基 

分 类 号：S668.9[农业科学—果树学]