纸色谱分离-荧光检测法测定人体尿液中的黄蝶呤  

Determination of Xanthopterin in the Human Urine by Paper Chromatography with Fluorescence Detection

作　　者：万益群[1] 张云伟[1] 郭岚[1] 

机构地区：[1]南昌大学化学系,南昌330047

出　　处：《分析科学学报》2004年第4期388-390,共3页Journal of Analytical Science

基　　金：江西省自然科学基金(No.012005);南昌大学校基金(2002年资助项目)

摘　　要：报道用丙醇-氨水(25％)-水(15:9:6,V/V)作展开剂,纸色谱分离人体尿液中的蝶呤类化合物,并利用黄蝶呤与其它蝶呤类化合物的荧光性质的差异来测定人体尿液中黄蝶呤的含量。黄蝶呤在0～0.6μg/10mL的范围内,其荧光强度与其荧光斑点的洗脱量呈良好的线性关系,从而建立了一种新的测定黄蝶呤的方法。该方法应用于人体尿液中黄蝶呤的测定,结果满意。The separation of pterins by paper chromatography using isopropanol-ammonia liquor-water (15:9:6, V/V) as developing solvent was studied in this article. Based on the difference of fluorescence property of xanthopterin from others, Xanthopterin was determined in the human urine. The fluorescence intensity of xanthopterin was proportional to the elution amount over the range of 0 to 0.6μg. The linear regression equation was Y=14.2934X-0.0742, with linear correlation coefficient of 0.9990. The proposed method was successfully applied to the determination of xanthopterin in the human urine samples.

关 键 词：纸色谱 荧光检测 尿液 黄蝶呤 

分 类 号：O657.39[理学—分析化学] Q586[理学—化学]