混合蛋白质体系中魟鱼和海兔肝铁蛋白释放铁的动力学研究  

STUDIES ON KINETICS OF IRON RELEASE FROM THE LIVER FERRITINS OF Dasyatics akajei AND Aplysia UNDER THE SYSTEM OF MIXED PROTEINS

作　　者：陈平[1] 黄河清[1] 颜利[1] 黄河宁[1] 杨天赐[1] 周常义[1] 

机构地区：[1]厦门大学生命科学学院福建省化学生物学重点实验室,厦门361005

出　　处：《生物物理学报》2004年第4期285-289,共5页Acta Biophysica Sinica

基　　金：国家自然科学基金项目(40276033);厦门科技项目(3502Z2001262)

摘　　要：分别制备含有魟鱼肝铁蛋白(liver ferritin of Dasyatisakajei, DALF)和海兔肝铁蛋白(Liver ferritin of Aplysia, ALF)的混合蛋白质体系。选用电子光谱技术和不同电子供体,研究在混合蛋白质体系中,DALF和ALF释放铁的动力学过程和规律。实验结果表明,采用Na2S2O4作为还原剂时,DALF以两相行为进行释放铁的反应;而选用抗坏血酸作为还原剂时,DALF却以一级反应动力学方式进行释放铁的反应,简化释放铁的过程。在混合蛋白质体系中且以抗坏血酸和Na2S2O4为电子供体时,ALF均以一级反应动力学过程进行释放铁的反应,认为某些蛋白质参与协助ALF释放铁反应,从而简化释放铁的过程。The mixed system of proteins of the liver ferritin of Dasyatis akajei (or Aplysia) and the other proteins was prepared by the technique of centrifugal method and temperature treatment. Under the system, kinetic processes and laws of iron release from the liver ferritins of Dasyatis akajei (DALF) and Aplysia (ALF) were studied by the technology of electronic spectra and the different electronic donor. The experimental results showed that biphasic behavior of iron release from the ferritin were observed while Na2S2O4 was used as a reducer. Moreover, another kinetics of first-order for iron release from the DALF was observed during vitamin C was as a reducer, it indicated that this process of iron release was simplified. Another system consisted of the mixed proteins and the ALF was used to study the kinetics of iron release while the vitamin C and Na2S2O4 were selected as electron donor, respectively. The kinetics showed that the ferritin released the iron as a form of first-order law, it indicated that some unknown proteins in the Aplysia liver participated in the reaction of iron release and resulted in the complex process of iron release to transferred into simplification.

关 键 词：铁蛋白 动力学释放铁 海兔和魟鱼肝 混合蛋白质体系 

分 类 号：Q6[生物学—生物物理学]