霍霍巴组培快繁技术体系研究 I.基本培养基与培养条件的优化  

Studies on the Technique System of Tissue Culture and Rapid Propagation of Jojoba I. Selection of Basic Medium and Culture Condition

作　　者：徐进[1] 王玉珍[1] 罗景兰[1] 刘小京[1] 

机构地区：[1]中国科学院遗传与发育生物学研究所农业资源研究中心

出　　处：《河南农业科学》2004年第8期21-24,共4页Journal of Henan Agricultural Sciences

基　　金：河北省国际科技合作计划项目资助 ( 0 3390 16 2D)

摘　　要：以霍霍巴为材料 ,研究了外植体的消毒、基本培养基、取材部位和培养条件等因素对组培苗生长的影响。结果表明 :采用 0 .1 %HgCl2 与 2 %NaClO 1∶1混合的消毒合剂处理外植体 8min ,灭菌成功率 99% ;改良MS培养基适合霍霍巴的分化培养 ;最适外植体为双腋芽茎段 ;培养条件 :温度 2 5～ 2 8℃ ,光照强度 30 0 0lx ,光照时间 1 2～ 1 4h/d ,pH 5 .5～ 6.0。The key techniques including explants disinfection, basic medium, suitable explant and culture condition were studied.By culturing the stems of Jojoba in vitro,the results showed as that the success percentage of disinfection reached 99% when the explants were treated by 1∶1 mixture of 0.1% HgCl 2 and 2% NaClO for eight minutes,the modified MS was suitable for Jojoba micropropagation.The optimal explants were stems with double axillary buds.All the cultures were kept under the temperature of 25-28 ℃,light intensity 3000 lx,light time for 12-14 h/d,and pH 5.5-6.0.

关 键 词：霍霍巴 基本培养基 培养条件 

分 类 号：S759.39[农业科学—森林经理学]