钝顶螺旋藻藻胆体的稳定性研究  

The study of the stability of phycobilisomes in algae,Spirulina platensis

作　　者：张熙颖[1] 刘鲁宁[1] 陈秀兰[1] 张玉忠[1] 周百成[1] 

机构地区：[1]山东大学生命科学学院微生物技术国家重点实验室,山东济南250100

出　　处：《海洋科学》2004年第8期38-42,46,共6页Marine Sciences

基　　金：国家高技术研究与发展计划(863计划)项目(2002AA302213);山东省科技发展计划项目(031070113)

摘　　要：根据室温荧光发射光谱表征 ,研究了藻胆蛋白浓度、离子强度、pH和葡聚糖等因素对钝顶螺旋藻(Spirulinaplatensis)藻胆体的稳定性的影响。藻胆蛋白浓度为0.6～1.2g/L时 ,钝顶螺旋藻藻胆体的室温荧光发射峰在676～681nm之间 ,此时藻胆体的稳定性强 ,不易解离。在低离子强度(<0.75mol/L)条件下 ,钝顶螺旋藻藻胆体易解离 ,解离速度随离子强度的递减而加快。钝顶螺旋藻藻胆体在 pH7时稳定 ,而在 pH5,6,8,9时只有轻微解离 ,表明藻胆体在较宽的 pH范围内保持相对稳定。在钝顶螺旋藻藻胆体溶液中加入10%的葡聚糖 ,保存30d后藻胆体依然保持完整 。T he effects of ionic strength,phycobiliprotein concentrations,pH and dextran on the stability of the phyˉcobilisomes in Spirulina platensis were studied by room temperature fluorescence emission spectra measurement.The phyˉcobilisomes with0.6～1.2g/L phycobiliprotein concentrations exhibited high stability and did not easily dissociate,The fluorescence emission maxima were between676nm and681nm.The phycobilisomes were unstable and readily dissociated into various subparticles under lowionic concentration(<0.75mol/L).The speed and the degree of dissociation were increased with the decrease of the ionic concentration.Phycobilisomes in buffer solution at pH7were stable and slightly dissociated.But at pH5,6,8or9,it showed that phycobilisomes exhibited stable within broad pH range.When10%dextran was added into the phycobilisomes solution,the phycobilisomes could keep intact for30days at20℃,it could be concluded that dextran had protection effect on the stability of phycobilisomes.

关 键 词：钝顶螺旋藻(Spirulina pltensis) 藻胆体 荧光谱 稳定性 

分 类 号：Q586[生物学—生物化学]