甲醛对DNA损伤的彗星实验研究  

STUDY ON THE FORMALDEHYDE-INDUCED DNA DAMAGE WITH COMET ASSAY

作　　者：李睿[1] 鲁志松[1] 乔琰[1] 姚汉超[1] 于非非[1] 杨旭[1] 

机构地区：[1]华中师范大学生命科学学院,武汉430079

出　　处：《实验生物学报》2004年第4期262-268,共7页Acta Biologiae Experimentalis Sinica

基　　金：国家"十五"科技攻关课题(2001BA704B01)经费资助~~

摘　　要：甲醛是一种遗传毒性物质。国内外学者的大量研究证实,甲醛可以引起DNA-DNA、DNA-蛋白质分子交联,但对于甲醛是否能够引起DNA 分子的断裂,学界却存在分歧。本实验以颊黏膜细胞作为实验材料,通过彗星实验对甲醛的遗传毒性——尤其是DNA 分子断裂作用进行了系统的研究。结果显示甲醛在较低浓度(5μmol/L,7.5μmol/L,10μmol/L)时具有断裂作用,在较高浓度(15μmol/L,30μmol/L,50μmol/L)时则具有交联作用。根据本实验的结果,本文还首次论证了甲醛断裂作用的断裂峰值(7.5μmol/L)。Formahlehyde is known as a genotoxic substance.Numerous studies have shownthat formaldehyde could induce DNA-DNA and DNA-protein crosslinks.However,scholars havedisagreed with each other on the formaldehyde-induced DNA strand breaks.We chose buccal cellsas materials to evaluate the genotoxieity of formaldehyde with comet assay—especially for DNAstrand breaks.The results showed that formaldehyde of low concentration induced DNA strandbreaks,while formaldehyde of high concentration induced DNA-DNA and DNA-protein crosslinks.According to our experimental results,we proposed the concept of the“peak point of break”atwhich formaldehyde induced DNA strand breaks most.

关 键 词：甲醛 DNA损伤 彗星实验 断裂峰值 颊黏膜细胞 遗传毒性 

分 类 号：X503.2[环境科学与工程—环境工程]