HBx蛋白与Hepsin共表达可促进HBV复制  

Co-expression of HBx and Hepsin Promotes Replication of HBV

作　　者：赵伟光[1] 张建林[1] 吴建国[1] 朱应[1] 李雁[1] 朱忠超[2] 刘志苏[2] 

机构地区：[1]武汉大学生命科学学院病毒学教育部重点实验室,湖北武汉430072 [2]武汉大学医学院中南医院,湖北武汉430071

出　　处：《中国病毒学》2004年第4期345-348,共4页Virologica Sinica

摘　　要：运用RT-PCR技术从原发性肝癌(Hepatocellularcarcinoma,HCC)患者的癌旁组织中扩增了Hepsin基因编码区序列,该序列已被克隆并构建了pCMV-tag-HS表达质粒。将pCMV-tag-HS表达质粒和表达HBx蛋白的载体共转染HepG2.2.1.5细胞,观察HBx蛋白和Hepsin蛋白相互作用对HBV病毒复制和病毒蛋白表达的影响。研究结果表明共表达HBx蛋白、Hepsin蛋白可以协同提高HBV病毒颗粒在培养液中的拷贝数,并提高HBV病毒蛋白HBs、HBe的表达水平。这说明HBx蛋白和Hepsin蛋白相互作用可以增强HBV病毒的复制。Entire ORF of Hepsin was isolated from adjacent live tissue of HCC with RT-PCR. Hepsin gene was inserted into expressing vector pCMV-tag-HS. The recombinant vector pCMV-tag-HS was co-transfected into liver tumor line Hep G2.2.1.5 with plasmid encoding HBx protein. The results indicated that co-expression of HBx and Hepsin increased the expressing level of viral proteins and the copy number of HBV, which implicated that the association of HBx and Hepsin promoted the replication of HBV.

关 键 词：RT-PCR技术 HBX蛋白 Hepsin蛋白 表达 HBV 复制 乙型肝炎病毒 

分 类 号：R373[医药卫生—病原生物学]