彭泽鲫两个雌核发育克隆的染色体组型分析  

Karyotypic Analysis from Two Clones of Gynogenetic Pengze Crucian Carp(Carassius auratus of pengze)

作　　者：刘良国[1] 赵俊[1] 陈湘粦[1] 董成稳[1] 崔淼[1] 

机构地区：[1]华南师范大学生命科学学院

出　　处：《Acta Genetica Sinica》2004年第8期780-786,共7页

基　　金：广东省自然科学基金 (编号 :0 3 15 2 5 );广东省科技攻关 (编号 :2 0 0 2C2 0 3 2 4) );广东省教育厅科学基金 (编号 :2 0 0 1-72 -0 12 1)资助项目~~

摘　　要：采用PHA和秋水仙素体内注射法直接制作肾细胞染色体标本 ,对彭泽鲫种群内两个不同雌核发育克隆的亲本进行染色体数目及组型分析。结果表明 ,彭泽鲫种群内的两个不同克隆存在染色体数目及组型差异 ,其中克隆H包含 6条超数染色体在内的染色体众数是 15 6 ,15 0条基本染色体的组型公式为 :4 2M +36SM +39ST +33T ,NF =2 2 8;克隆L包含 12条超数染色体在内的染色体众数是 16 2 ,15 0条基本染色体的组型公式为 :36M +45SM +33ST +36T ,NF =2 31。Pengze crucian carp (Carassius auratus of pengze),one of the most popular cultural fishes in China,is an endemic bisexual population with natural gynogenetic reproduction mode. Just like Silver crucian carp,investigation on the karyotype of Pengze crucian carp is important to understanding the diversity and the evolution of the unisexual vertebrates. Two distinguishable gynogenetic clones of Pengze crucian carp,named clone H and clone L,respectively,were detected recently. In this study,the chromosomes number and karyotype of the two clones were analyzed using chromosomes of kidney cell-PHA culture in vivo prepared by flame-drying technique. The results show that the chromosome numbers and karyotypes of the two clones are different. Clone H has 156 chromosomes in its karyotype with 42 metacentric,36 submetacentric,39 subtelocentric,33 telocentric,and six supernumerary chromosomes; the karyotype of clone L contains 162 chromosomes with 36 metacentric,45 submetacentric,33 subtelocentric,36 telocentric,and twelve supernumerary chromosomes. The karyotype formula of 150 basic chromosomes of clone H is 3n=42M+36SM+39ST+33T,NF=228;and the karyotype formula of 150 basic chromosomes of clone L is 3n= 36M+45SM+33ST+36T,NF=231. The discovery of the two different gynogenetic clones indicates that the genetic diversity also exists in the Pengze crucian carp,similar to that in the Silver crucian carp.

关 键 词：彭泽鲫 雌核发育 染色体组型 超数染色体 

分 类 号：S917[农业科学—水产科学]