家蚕AFLP分子连锁图谱的构建及绿茧基因定位  

Construction of AFLP Molecular Markers Linkage Map and Localization of Green Cocoon Gene in Silkworm (Bombyx mori)

作　　者：赵爱春[1] 鲁成[1] 李斌[1] 普孝英[1] 周泽扬[1] 向仲怀[1] 

机构地区：[1]西南农业大学蚕学与生物技术学院

出　　处：《Acta Genetica Sinica》2004年第8期787-794,共8页

基　　金：国家高技术研究发展计划 ( 863计划 )项目 (编号 :2 0 0 2AA2 110 11)资助~~

摘　　要：利用改进的AFLP技术 ,对家蚕品系C10 0 和大造的回交一代BC1群体进行连锁图谱的构建。经 2 8对引物组合的选择性扩增 ,共获得了 395 6条带 ,平均每对引物产生 14 1 3条带 ,获得多态性带只有 10 18条 ,多态性带的比率为 2 5 7%。其中 6 93(6 8 1% )个多态性位点符合 1∶1孟德尔分离比例。利用Mapmaker/Exp 3 0软件进行连锁分析 ,构建了一张含有 4 0 8个标记位点、33个连锁群、总图距为 36 76 7cM的连锁图谱 ,并将绿茧基因定位在该图谱的 2 2连锁群上 ,表明该连锁群与家蚕经典遗传学的第Based on an improved method of AFLP,AFLP markers were employed for construction of a linkage map and localization of Gc gene used a set of 44 backcross lines(BC 1) of silkworm(Bombyx mori) as a mapping population.In this work,all together 3 956 bands were obtained by 28 pairs of primers and 141.3 bands each primer pair on average.Among them 2 836 bands were in good agreement with the segregation pattern.A total of 1 018(25.7%) polymorphic AFLP markers were detected.The 693(68.1%) of polymorphic markers with 1∶1 segregation ratio (P≤0.05) were obtained.Furthermore,The analytical model was based on the backcross type and the parameters were set as following:LOD=3.0,maximum recombination value of 0.20 and use the command ‘group’,‘compare’,‘try’,‘map’ and ‘ripple’ to construct the linkage maps.407 of the 693 loci were χ~2 tested in agreement with 1∶1 segregation were divided into 33 linkages by Mapmaker/Exp(Version 3.0),with a total map distance of 3 676.7 cM and a mean distance of 9.1 cM between markers.The morphological gene Gc was located between L-P4T6-107 and L-PT6T4-84 on linkage group 22.In addition,286 markers were not included in the linkage groups.The efficiency of loci mapping was 58.7%.Among the 33 linkage groups,the morphological marker Gc classically localized on linkage group 15 was relocated on linkage group 22 on the map,suggesting that this molecular linkage group corresponds to linkage group 15 on the linkage map based on morphological characters.All these have laid an important base for the marker assisted breeding of the silkworm.

关 键 词：家蚕 分子连锁图谱 AFLP 绿茧 

分 类 号：S881.2[农业科学—特种经济动物饲养] Q755[农业科学—畜牧兽医]