猪皮肤成纤维细胞PERV体外和体内感染性的研究  

Studies on the Infectivity by Porcine Endogenous Retrovirus with Porcine Skin Fibroblast in vitro and in vivo

作　　者：丁生财[1] 陈意生[1] 魏泓[2] 商海涛[2] 刘萍[3] 李洪艳[3] 

机构地区：[1]第三军医大学西南医院病理学研究所,重庆400038 [2]第三军医大学实验动物中心,重庆400038 [3]第三军医大学新桥医院肝胆外科,重庆400037

出　　处：《Acta Genetica Sinica》2004年第8期813-818,共6页

基　　金：国家"973"项目 (国家重点基础研究发展规划项目 )"动物遗传育种与克隆的分子生物学基础研究"子项目课题资助 (编号 :G2 0 0 0 0 1610 6)~~

摘　　要：为了解猪皮肤成纤维细胞PERV在体外和体内的感染性 ,通过建立猪皮肤成纤维细胞系 ,将所建细胞系与人胚胎肾 2 93细胞体外共培养 ,并移植于严重联合免疫缺陷鼠 (SCID鼠 )皮下进行猪皮肤成纤维细胞PERV的体外和体内感染性实验。结果表明 ,猪皮肤成纤维细胞与人胚胎肾细胞共培养过程中 ,猪内源性逆转录病毒感染人胚胎肾细胞 ,进一步证实和拓宽了猪细胞PERV感染人细胞的范畴 ;猪皮肤成纤维细胞移植SCID鼠皮下后 ,导致SCID鼠发生猪细胞微嵌合 (78 5 7% )和PERV在体内感染 (85 71% )并且波及远离移植部位的多种组织或器官 ,但是并未检测出SCID鼠组织中表达PERVenvRNA。这就证实了猪皮肤成纤维细胞PERV的体外感染性和在小鼠体内的感染性 ,但未能找到PERV在体内活跃复制的明显证据。因而 。To understand the infectivity by porcine endogenous retrovirus with porcine skin fibroblast cell in vitro and in vivo, porcine skin fibroblast cell established by our laboratory were co-cultured with neo/HEK293 cell for the infection of PERV in vitro, and were subcutaneously transplantated to SCID (severe combined immuno-deficiency) mice for the infection of PERV in vivo, laying the foundation for valuation of biologic safety of xenotransplantation. The event of neo/HEK293 cells infected by PERV occurred during co-culture of porcine skin fibroblast cells with neo/HEK293 cells, expanding the rang of the infection of porcine endogenous retrovirus. After pig cells transplantated subcutaneously in SCID mice, the microchimerism (78.57%) of pig cells occurred widely, and there was phenomena of integration of PERV provirus (85.71%) in several organs or tissues remote from the injected sites, indicating infection of PERV in SCID mice in vivo. yet, there is no evidence of active viral replication in analysis of PERV env RNA of these tissues or organs. The infectivity of porcine endogenous retrovirus with porcine skin fibroblast cells in vitro and in vivo were demonstrated in this study, and there is no evidence of active viral replication of PERV in vivo. It is an important event of biologic safety which attention must be paid to potential hazard of infectious interspecies transmission of PERV during xenotransplantation.

关 键 词：猪内源性逆转录病毒 异种移植 感染 成纤维细胞 生物安全性 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]