诱导突变高粱愈伤组织初探  

Preliminary Probe Into Callus Inducement of Sorghum Mutant

作　　者：刘明[1] 赵琦[2] 王小菁[3] 赵玉锦[4] 童哲[4] 

机构地区：[1]首都师范大学,北京100037 [2]首都师范大学生物系,教授北京100037 [3]华南师范大学,广州510631 [4]中国科学院植物研究所,北京100093

出　　处：《生物技术通报》2004年第4期47-52,共6页Biotechnology Bulletin

基　　金：国家自然科学基金资助项目 ( 3 0 170 5 5 8)

摘　　要：本实验以突变体高粱的不同外植体 ,成熟种子、成熟胚、茎尖、和幼胚等为材料 ,诱导愈伤组织。经出愈率和生长状况观察 ,幼胚最好 ,成熟胚较好 ,成熟种子和茎尖略差。对于茎尖 ,2 ,4 D与KT搭配较好 ;成熟胚而言 ,不加细胞分裂素 ,加适量 2 ,4 D浓度效果较好 ,幼胚愈伤组织诱导和培养不需要细胞分裂素 ,加浓度为 2 .0mg/L2 ,4 D效果较好 ;若加KT ,同时要提高 2 ,4 D浓度 ;成熟种子培养 。This was the first time that we carried out the experiment on callus inducement of sorghum mutant . Seeds of pure breeding sorghum were treated by Chinese Reversal Man-made Satellite . We studied on the influences of the culture medium on the callus inducement and culture by using different parts of sorghum as experimental material. This paper deals with systematic studies on initiation ,maintenance and plant regeneration of callus . Good callus inducement was obtained from the cultures of four types of explants including seed, mature embryo,young embryo,and shoot tip . Among the factors which can influence the callus for formation and plant regeneration of sorghum mutants,we focused on the the most crucial elements 2,4-D and KT in Murashige-Skoog(MS) medium.We found the suitable contion for the callus formation and also the plant regeneration afterwards.

关 键 词：高粱 愈伤组织 突变体 外植体 组织培养 再生 

分 类 号：S514[农业科学—作物学]