杆状病毒表面展示系统的研究进展  

Progress in Baculovirus Surface Display System

作　　者：林旭瑷[1] 陈寅[1] 张志芳[1] 何家禄[1] 沈桂芳[2] 

机构地区：[1]中国农业科学院蚕业研究所,镇江212018 [2]中国农业科学院生物技术研究所,北京100081

出　　处：《生物技术通报》2004年第4期5-9,共5页Biotechnology Bulletin

基　　金：国家自然科学基金 ( 3 9970 5 71;3 0 2 710 0 7)资助项目

摘　　要：杆状病毒表面展示系统是近几年发展起来的一种新的真核展示系统 ,通过在病毒衣壳蛋白gp6 4插入外源肽、二者融合表达或与特异性的锚定部位结合 ,在病毒表面进行融合表达而筛选出目的活性肽或蛋白。可用来展示需糖基化、二硫键异构化等翻译后修饰才表现功能活性的复杂真核蛋白及构建多肽文库、抗体库等。本文简述了该技术的原理、研究进展、应用及发展前景等。可以预见 。Baculovirus surface display system is a new eukaryotic display technology developed in recent years, which can be used for reconstructing and screening the interested active polypeptide or protein, through fused to a second copy of the entire coat protein of baculovirus, gp64 or alternatively to the membrane anchor domain of gp64, simultaneous expression of the fusion construct and the native gp64 envelope protein results in packaging of both proteins into the viral coat and its presence on the surface of baculovirus-infected insect cells and/or budded virions. Especially in the use of displaying complex eukaryotic proteins requiring post-translational processing including glycosylation and efficient folding for functional activity. In this paper we mainly described the basis of this technology, its development, its applications and perspective. Therefore, the development of baculovirus surface display technology will bring profound influence on life science and related subjects.

关 键 词：杆状病毒 表面展示系统 真核生物 融合表达 生命科学 

分 类 号：Q78[生物学—分子生物学]