玉米杂交种SSR标记纯度鉴定方法研究  

Study on purity testing methods for maize hybrids using SSR markers

作　　者：谭振馨[1] 李汝玉[1] 李群[1] 颜廷进[1] 张文兰[1] 宋国安[1] 侯淑贞[1] 

机构地区：[1]山东省农业科学院作物研究所,山东济南250100

出　　处：《山东农业科学》2004年第4期8-11,共4页Shandong Agricultural Sciences

摘　　要：以常规种子贮藏蛋白质电泳技术难以鉴定纯度的4个玉米杂交种、21个自交系和9对玉米SSR位点引物为材料,运用SSR分子标记技术分别筛选出适合这些不同杂交种纯度鉴定的SSR位点引物,建立了一套仪器设备要求相对较低、程序简单、较为快速地利用SSR标记进行玉米杂交种纯度鉴定的技术规程,并对利用SSR标记进行玉米杂交种纯度鉴定的可行性进行了分析。In this study, 9 SSR primer pairs were screened to search for the SSR loci that are suitable for testing the purities of 4 maize hybrids, which lack a reliable laboratory method for detecting a large proportion of variation in SSR allele sizes. Polymorphic SSR loci have been found that could be applied to test the purities of these 4 hybrids. A procedure featuring inexpensive equipment and ease of use has been set up for testing maize hybrid purity using SSR marker. The feasibility of applying SSR markers to maize hybrid purity using this procedure was discussed .

关 键 词：玉米杂交种 纯度鉴定 SSR标记 

分 类 号：S-03[农业科学]