两种缘毛类纤毛虫胞质Hsp70基因序列的克隆及其系统发育分析  

Phylogenetic Analysis of Peritrichs Inferred from Cytosolic Hsp70 Gene Partial Sequences

作　　者：傅诚杰[1] 缪炜[1] 沈韫芬[2] 万明亮[2] 

机构地区：[1]中国科学院东湖湖泊生态系统试验站,湖北武汉430072 [2]中国科学院水生生物研究所,湖北武汉430072

出　　处：《Zoological Research》2004年第4期298-303,共6页动物学研究（英文）

基　　金：中国科学院创新工程基金资助项目 ( 2 2 0 2 0 7) ;东湖湖泊生态系统试验站开放基金资助项目 ( 2 0 0 40 5 )

摘　　要：克隆得到 2种缘毛类纤毛虫———钟形钟虫 (Vorticellacampanula)和螅状独缩虫 (Carchesiumpolyp inum)的胞质Hsp70基因部分序列 ,长度均为 4 38bp ,编码 14 6个氨基酸。以细菌为外类群 ,利用最大似然法和邻接法构建包括其他 5种纤毛虫在内的共 2 6个物种的Hsp70基因氨基酸序列系统发育树 ,其拓扑结构显示 :V campanula和C polypinum聚在一起 ,并与另 2种寡膜纲的嗜热四膜虫 (Tetrahymenathermophila)及草履虫(Parameciumtetraurelia)聚为姊妹枝 ,提示了缘毛类纤毛虫为单系 ,且隶属于寡膜纲的系统发育地位。Cytosolic Hsp70 genes of two peritrichous ciliates (Vorticella campanula & Carchesium polypinum) were partially sequenced and the lengths are 438 bp,both encoding 146 amino acids.Using bacteria as the outgroup,the phylogenetic trees were constructed based on cytosolic Hsp70 amino acid sequences by maximum-likelihood (ML) and neighbor-joining (NJ) methods.The topology that V.campanula clustered with C.polypinum,and then formed a sister clade with another two species in Oligohymenophorea (Tetrahymena thermophila & Paramecium tetraurelia),suggested the monophyly of the peritrichs and their phylogenetic placement in the class Oligohymenophorea.

关 键 词：缘毛类纤毛虫 胞质 HSP70基因 克隆 系统发育 

分 类 号：Q785[生物学—分子生物学]