Constructing Competitive Reverse Transcription Polymerize Chain Reaction Inter-Reference of PC mRNA by Intron Approach  

作　　者：XUChuang XIACheng LIUGuo-wen WANGZhe JIANGYu-fu ZHANGNai-shen~ FUShi-xin 

机构地区：[1]FacultyofMilitaryVeterinaryMedicine,QuartermasterUniversityofChinesePeople＇sLiberationArmy,Changchun130062,P.R.China

出　　处：《Agricultural Sciences in China》2004年第7期548-552,共5页中国农业科学（英文版）

基　　金：supported by the National Natural Science Foundation of China(30230620).

摘　　要：Inter-reference of competitive reverse transcription polymerase chain reaction (RT-PCR)was constructed by intron method to detect the change of PC mRNA level in the pathway ofcarbohydrate metabolism. The experiment based on the principle that 81bp intron sequencewas deleted in PC mRNA compared with PC DNA sequence. The 466bp competitive DNA templaterecombinant plasmid of PC mRNA was successfully built by a pair of primer and was clonedonce, PC DNA and PC mRNA could be inter-referred each other. The intron approach used inthe experiment has broken through the traditional method of constructing competitivetemplate.Inter-reference of competitive reverse transcription polymerase chain reaction (RT-PCR)was constructed by intron method to detect the change of PC mRNA level in the pathway ofcarbohydrate metabolism. The experiment based on the principle that 81bp intron sequencewas deleted in PC mRNA compared with PC DNA sequence. The 466bp competitive DNA templaterecombinant plasmid of PC mRNA was successfully built by a pair of primer and was clonedonce, PC DNA and PC mRNA could be inter-referred each other. The intron approach used inthe experiment has broken through the traditional method of constructing competitivetemplate.

关 键 词：PC Competitive template INTRON Quantitative RT-PCR CLONE 

分 类 号：Q78[生物学—分子生物学] Q55