大孔树脂吸附和纯化蒲黄总黄酮的工艺研究  

Absorption and Purification of the Total Flavonoids of Pollen typhae with Macro Resin

作　　者：傅大煦[1] 程小卫[2] 王玮[2] 陈家宽[2] 周铜水[2] 

机构地区：[1]上海新药研究开发中心,上海201203 [2]复旦大学生命科学学院,上海200433

出　　处：《华东理工大学学报（自然科学版）》2004年第4期462-464,共3页Journal of East China University of Science and Technology

摘　　要：以主成分香蒲新苷及异鼠李素-3-O-新橙皮糖苷含量为指标,研究了HZ802非极性大孔树脂吸附和纯化蒲黄总黄酮的工艺条件。结果表明HZ802非极性大孔树脂对蒲黄总酮有良好的吸附纯化性能,其优化的工艺条件为:样品溶液的浓度为50mg/mL(生药量/体积);树脂用量为生药量的1.5倍(质量比);纯化先用2倍树脂柱体积的水,再用2倍树脂柱体积φ=0.2的乙醇;总黄酮的洗脱用2倍树脂柱体积φ=0.5的乙醇;吸附和洗脱流速均为2mL/min。An optimum technique for absorbing and purifying the total flavonoids of Pollen typhae with HZ802 non-polarity macro resin was developed, using the contents of typhaneoside and isorhamnetin-3-O-neohesperidoside as the evaluating criteria. The results showed that HZ802 non-polarity macro resin was suitable for absorbing and purifying the total flavonoid of Pollen typhae. The optimum technical operations were as follows:concentration of the sample was 50 mg/mL(weight of the raw material/volume); volume of the resin was 3 times weight of the raw material; purified with distilled water (2 times the volume of the resin), and then, with 20% ethanol (2 times the volume of the resin); 50% ethanol (2 times the volume of the resin) was used to elute the total flavonoids; both the current velocity of absoring and eluting were 2 mL/min.

关 键 词：蒲黄总黄酮 HZ802非极性大孔树脂 香蒲新苷 异鼠李素-3-O-新橙皮糖苷 

分 类 号：O643.3[理学—物理化学]