雌性大鼠心内神经节细胞中ER与NOS_1的共存  

Coexistence of ER and NOS_1 in Intracardiac Ganglionic Cells of Female Rat

作　　者：陈小武[1] 祝善乐[2] 范玉华[2] 赵小明[2] 

机构地区：[1]咸宁学院医学院人体解剖学教研室,湖北咸宁437100 [2]华中科技大学同济医学院解剖系

出　　处：《咸宁学院学报（医学版）》2004年第2期81-82,86,共3页Journal of Xianning Univarsity(medical Sciences)

摘　　要：目的探讨雌性大鼠心内神经节细胞中ER与NOS1共存的可能性。方法采用免疫组织化学SABC法双重标记技术 ,用鼠抗ER血清和兔抗NOS1血清分别孵育切片 ,葡萄糖氧化酶 硫酸镍胺 DAB/AP red试剂盒呈色。结果在ER/NOS1双重免疫组织化学反应下可见三种细胞 :①NOS1单标细胞 :胞浆为NOS1 IR阳性 ,反应产物鲜红色 ,胞核未见着色 ;②ER单标细胞 :胞核为ER IR阳性 ,胞浆未见明显染色。③ER/NOS1双标细胞 :胞核为ER IR阳性 ,反应产物黑蓝色 ,胞浆为NOS1 IR阳性 ,反应产物鲜红色。ER/NOS1双标细胞约占NOS1 IR阳性神经元总数的 19%、占ER IR阳性神经元总数的 34%。结论雌性大鼠心内神经节细胞中ER与NOS1共存 ,部分ER IR阳性神经元为NO能神经元。Objective In order to investigate the coexistence of estrogen receptor(ER) and nitric oxide synthase 1 (NOS 1 ) in intracardiac ganglionic neurons. Methods Double labelling immunohistochemical SABC technique was utilized :The atrial sections of rats were separately incubated with mouse anti-ER serum and rabit anti- NOS 1 serum. Results The ER immunoreaction showed dark-blue or brown products in the nuclei.The NOS 1-immunoreaction showed pink products in the cytoplasm.There are three kinds of dyed cells:① NOS 1-IR single labelled cells;②ER-IR single labelled cells;③ER/ NOS 1 double labeled cells. The ER/ NOS 1 double labeled cells account for about 19 percent of all the NOS 1-IR postive neurons and for about 34 percent of all the ER-IR postive neurons. Conclusion The subpopulation of ER-IR neurons are NO-energic neurons in the intracardiac ganglia in female rats.

关 键 词：心内神经节 雌激素受体 ER NOS1 免疫组织化学 雌性大鼠 

分 类 号：Q579.13[生物学—生物化学]