胆红素对正己烷致体外外周血淋巴细胞DNA损伤的拮抗作用  

Protective effect of bilirubin against the damage of n-hexane on DNA of peripheral lymphocytes

作　　者：黎燕[1] 邰昌松[2] 杨青[1] 王绵珍[1] 沈惠麒[3] 

机构地区：[1]四川大学华西公共卫生学院劳动卫生教研室,四川成都610041 [2]深圳市宝安区卫生防疫站,广东深圳518101 [3]北京大学公共卫生学院劳动卫生与环境卫生学系,北京100083

出　　处：《中国工业医学杂志》2004年第3期152-153,162,共3页Chinese Journal of Industrial Medicine

摘　　要：目的　研究胆红素 (bilirubin)对正己烷 (n hexane)致DNA损伤的影响。方法　应用单细胞凝胶电泳(SCGE)技术和彗星图像分析系统 ,检测不同浓度正己烷对人外周血淋巴细胞DNA损伤 ,同时观察 10 μmol/L胆红素对正己烷所致的DNA损伤的保护作用。结果　尾长、尾 /头 (长 )、尾 /头 (光强 )、迁移率、尾DNA %、Olive尾矩等指标与正己烷浓度存在明显的剂量 效应关系 ,发现胆红素能抑制正己烷的DNA断裂能力。Objective To study the effect of bilirubin on DNA damage due to n-hexane.Method Single cell gel electrophoresis assay was used to observe DNA damage due to n-hexane exposure at different doses and the protective effect of bilirubin.Result There was dose-response relationship between n-hexane exposure levels and the indexes including tail length,tail /head(length and intensity),migration rate,tail% DNA,and Olive tail moment etc.,and bilirubin showed a protective effect against the damage on single strand DNA in peripheral lymphocytes.Conclusion n-Hexane could cause DNA damage in peripheral lymphocytes and the damage could be inhibited by bilirubin.

关 键 词：正己烷 单细胞凝胶电泳 DNA损伤 胆红素 

分 类 号：O623.11[理学—有机化学] R394.6[理学—化学]