β-环糊精修饰区带毛细管电泳法快速测定染料木甙、染料木素、山柰酚和芦丁  

Determination of Ggenistin, Genistein, Kaemferol and Rutin in Fructus Sophorae by β-CD Modified Capillary Zone Electrophoresis

作　　者：吕元琦[1] 袁倬斌[1] 

机构地区：[1]中国科学院研究生院应用化学研究所,北京100039

出　　处：《生命科学仪器》2004年第3期30-30,32,共2页Life Science Instruments

基　　金："十五"科技攻关重大项目(2001BA210A04-7);国家自然科学基金(20175025);国家自然科学基金分析化学重点基金(20235010);电分析化学国家重点实验室基金;"九五"科技攻关重点项目(96-A23-01-06)的资助

摘　　要：A method was developed to quickly determine genistin, genistein, kaempferol and rutin in Fructus sophorae by β -CDmodified capillary zone electrophoresis. Based on the investigation of the effects of buffer pH and concentration, β -CD concentration,SDS concentration, capillary temperature, the analytical conditions were optimized. Under the optimized conditions, the three analytes were well separated in less than 6 min. A good linear relationship between the peak area and concentration was found in the 2 ～ 400g/mL,2 ～ 300g/mL 2 ～ 200g/mL and 5 ～ 500g/mL concentration range for genistin, genistein, kaempferol and rutin, respectively. The detection limits were 0.8g/mL, 0.6g/mL, 0.6g/mL and 1.4g/mL for genistin, genistein, kaempferol and rutin, respectively.A method was developed to quickly determine genistin, genistein, kaempferol and rutin in Fructus sophorae by β-CD modified capillary zone electrophoresis. Based on the investigation of the effects of buffer pH and concentration, β-CD concentration, SDS concentration, capillary temperature, the analytical conditions were optimized. Under the optimized conditions, the three analytes were well separated in less than 6 min. A good linear relationship between the peak area and concentration was found in the 2-400g/mL, 2-300g/mL 2-200g/mL and 5-500g/mL concentration range for genistin, genistein, kaempferol and rutin, respectively. The detec-tion limits were 0.8g/mL, 0.6g/mL, 0.6g/mL and 1.4g/mL for genistin, genistein, kaempferol and rutin, respectively.

关 键 词：β-环糊精修饰区带 测定 染料木甙 染料木素 山柰酚 芦丁 毛细管电泳法 黄酮类化合物 抗氧化剂 

分 类 号：R917[医药卫生—药物分析学]