补肾壮骨方对去卵巢骨质疏松大鼠IL-6骨表达的影响  

Effect of Bushen Zhuanggu Prescription,A Traditional Chinese Medicine,on IL-6 Expression in Ovariectomized Rats

作　　者：颜灿群[1] 陈友香[1] 夏振信[1] 

机构地区：[1]武汉大学中南医院中西医结合科,武汉430071

出　　处：《武汉大学学报（医学版）》2004年第4期440-443,T001,共5页Medical Journal of Wuhan University

基　　金：湖北省卫生厅重点项目基金 (基金号 980 1)

摘　　要：目的 :观察补肾壮骨方对实验性骨质疏松大鼠骨密度 (BMD)、白细胞介素 6 (IL 6 )骨表达的影响 ,进一步探讨补肾壮骨方抗骨质疏松的作用机理。方法 :使用补肾壮骨方对切除卵巢的雌性成年Wistar大鼠进行不同剂量的药物治疗 ,并与正常对照组、模型组和尼尔雌醇治疗组的大鼠进行了对比 ,采用免疫组化的方法 ,检测并比较各组骨组织IL 6骨表达。结果 :补肾壮骨方各剂量组和尼尔雌醇组可使骨质疏松大鼠的股骨、腰椎和全身的骨密度明显增加 ,且骨组织中IL \|6的阳性表达明显弱于模型组 ,有统计学意义 (P <0 .0 5 )。结论 :补肾壮骨方抗骨质疏松的作用可能是通过减弱骨组织中的IL 6表达 ,增加骨密度来发挥作用的。Objective: To probe the effect of Bushen Zhuanggu prescription(BSZHG),a traditional Chinese medicine,on experimental osteoporosis in rats. Methods: Bone mineral density (BMD) was measured and the expression of interleukin-6(IL-6) in bone was observed. Ovariectomized female Wistar rats were treated with BSZHG in different doses,and compared with groups of normal control,model and nilestrial-treated rats. Bone IL-6 expression during different groups rats were investigated and compared with by means of immunohistochemistry. Results: BMD of femur,lumbar spine and body increased in BSZHG groups of different doses. The positve expression of IL-6 in traditional Chinese medicine and normal control group was obviously weaker than that in the model control group. Conclusion: BSZG could significantly inhibit IL-6 expression.

关 键 词：补肾壮骨方 去印象大鼠 骨密度 白细胞介素6 骨质疏松 

分 类 号：R681[医药卫生—骨科学]