口腔黏膜上皮细胞及成纤维细胞与PGA的生物相容性研究  

Evaluation of the biocompatibility of PGA with oral keratinocyte & fibroblast

作　　者：黄鹤[1] 周曾同[1] 杨光辉[2] 崔磊[2] 刘伟[2] 曹谊林[2] 

机构地区：[1]上海第二医科大学附属第九人民医院口腔内科,上海200011 [2]上海市组织工程研究与开发中心

出　　处：《临床口腔医学杂志》2004年第7期387-390,共4页Journal of Clinical Stomatology

基　　金：上海市科委基金资助 (99XD1 4 0 3);上海第二医科大学博士点建设基金资助 (2 0 0 3 -2 0 0 4 )

摘　　要：目的 :观察口腔黏膜上皮细胞与成纤维细胞在聚羟基乙酸 (PGA)上的粘附生长情况 ,在体外形成活的黏膜样组织的能力。方法 :口腔黏膜上皮细胞无血清、无饲养层体外培养、扩增至第二代 ,与PGA混合培养形成细胞 -生物材料复合物 ;口腔黏膜成纤维细胞体外培养、扩增至第二代 ,与PGA混合培养形成细胞 -生物材料复合物 ;①光镜、扫描电镜下观察两种细胞分别与PGA的粘附生长情况 ;②用MTT法检测口腔黏膜上皮细胞及成纤维细胞在PGA上的增殖情况 ,绘制细胞生长曲线。结果 :口腔黏膜上皮细胞与成纤维细胞能够在PGA上粘附生长 ,其生长增殖能力优于平皿培养。结论 :PGA与口腔黏膜上皮细胞或成纤维细胞具有良好的生物相容性 ,其降解产物无毒 。Objective:The aim of this study was to evaluate the biocompatibility of PGA with oral keratinocyte & fibroblast and to improve cell!seeding methods for developing tissue engineered oral mucosa.Method: Human oral keratinocytes and fibroblast were seeded on PGA mesh separately. 1) Observe cell attachment and tissue development on the PGA scaffolds by light microscopy and electron microscopy. 2) In addition, MTT was also performed to document growth of oral mucosa keratinocyte and fibroblast.Result: Cells can attach and grow on the PGA scaffolds, The fibroblasts and keratinocytes were also found to grow faster on PGA fibers than on culture dish.Conclusion: PGA has excellent biocompatibility with oral mucosa keratinocytes and fibroblast. It's feasible to use it to develop a new functional tissue in vitro.

关 键 词：生物工程 上皮细胞 成纤维细胞 MTT 

分 类 号：Q813.1[生物学—生物工程]