酶联免疫吸附试验和聚合酶链反应检测生殖器标本中的单纯疱疹病毒  

Detection of herpes simplex virus with enzyme immunoassay and polymerase chain reaction

作　　者：张津萍[1] 张树文[1] 钟铭英[1] 尤永燕[1] 龚匡隆[1] 

机构地区：[1]中国医学科学院中国协和医科大学皮肤病研究所,江苏南京210042

出　　处：《中国艾滋病性病》2004年第3期202-203,211,共3页Chinese Journal of Aids & STD

摘　　要：目的　评价酶联免疫吸附试验 (ELISA)诊断生殖器疱疹的临床应用价值。方法　对 12 0例具有生殖器皮肤、粘膜损害的病人 ,采用ELISA和分型聚合酶链反应 (PCR)检测其生殖器标本中的单纯疱疹病毒 (HSV) ,两种试验结果不符合者采用第二种PCR检测。结果　 12 0例受检者中 ,ELISA检出 4 9例HSV阳性 ,分型PCR检出 5 0例阳性。单纯HSV 1感染者占生殖器疱疹的 10 0 % ,单纯HSV 2感染者占 80 0 % ,HSV 1和HSV 2混合感染者占 10 0 %。在 12 0例标本中 ,10 7例两种试验结果是符合的 ,13例结果不符合。与“扩大金标准”比较 ,ELISA的敏感性、特异性、阳性预期值分别为 92 0 %、95 7%、93 8% ,分型PCR的敏感性、特异性、阳性预期值分别为 94 0 %、95 7%、94 0 %。结论　ELISA法检测HSV感染 ,其敏感性和特异性高 ,方便、快速 ,尤其适合大批量样本的检测 ,值得在临床应用。Objective To evaluate the performance of a commercial available ELISA kit and polymerase chain reaction(PCR) for the diagnosis of genital herpes.Methods Genital specimens were tested with ELISA and typing PCR in 120 patients with genital lesions.The discrepant results were resolved by application of other PCR with different primers. Results Among the 120 patients HSV was detected in 49 by ELISA and 50 by typing PCR.Ten percent of genital herpes were caused by HSV-1 alone,80% by HSV-2 alone,and 10% by mixed HSV-1 and HSV-2,respectively.Of the 120 specimens,107 showed coherent results by both tests,and 13 failed to do so.In comparison with expanded gold criteria,the sensitivities,specificities and positive predictive values of ELISA and PCR were 92.0% and 94.0%,95.7% and 95.7%,93.8% and 94.0%,respectively.Conclusion It is indicated that ELISA is simple,rapid,high sensitive and specific for the diagnosis of genital herpes;the method is suitable for the testing of large batches of clinical specimens and is recommended for clinical application.

关 键 词：生殖器疱疹 酶联免疫吸附试验 聚合酶链反应 

分 类 号：R322.6[医药卫生—人体解剖和组织胚胎学] R752.11[医药卫生—基础医学]