环孢素A气雾吸入对哮喘大鼠气道炎症的影响  

Effect of inhaled cyclosporin A on antigen-induced airway inflammation in asthmatic rats

作　　者：陈莹[1] 谢强敏[2] 杨秋火[2] 陈季强[2] 

机构地区：[1]浙江大学医学院附属第一医院肾脏病中心,浙江杭州310003 [2]浙江大学医学院国家食品药品监督管理局浙江呼吸药物研究重点实验室,浙江杭州310031

出　　处：《药学学报》2004年第7期486-490,共5页Acta Pharmaceutica Sinica

基　　金：国家重点科技 (攻关 )项目 ( 96-90 1-0 5 -2 69)

摘　　要：目的　研究环孢素A(CsA)气雾吸入对抗原诱导的大鼠过敏性气道炎症的作用。方法　用卵白蛋白 (OA)致敏大鼠 ,2周后气雾吸入CsA(5 ,10 ,2 0g·L- 1 ) ,每天 1次 ,连续 7d。大鼠致敏后d 2 0和d 2 1用OA(10g·L- 1 ,每天 1次 )攻击 ,观察第 2次OA攻击 2 4h后支气管肺泡灌洗液及外周血中嗜酸性粒细胞的数量和支气管肺组织病理学改变情况 ,测定支气管肺泡灌洗液中TNF α含量。结果　CsA气雾吸入能明显降低支气管肺泡灌洗液及外周血中嗜酸性粒细胞的数量 ,减轻肺组织中炎症细胞特别是嗜酸性粒细胞的浸润 ,减轻组织水肿及上皮损伤等气道炎症状况 ,降低支气管肺泡灌洗液中TNF α含量。结论　CsA气雾吸入对大鼠过敏性气道炎症具有抑制作用 ,其作用机制与细胞因子TNFAim To investigate the effect of inhalation of cyclosporin (CsA) on antigen-induced airway inflammation in Sprague-Dawley rats. Methods Rats were sensitized with antigen (ovalbumin, OA). After two weeks, the sensitized rats were pretreated with aerosol CsA (5, 10, 20 g·L -1),once per day for 7 days. Then, the sensitized rats were challenged with OA (10 g·L -1,once per day) for 2 days at day 20 after sensitization. The number of eosinophils in bronchoalveolar lavage fluid (BALF) and peripheral blood, histological changes of lung tissue, and TNF-α content in BALF were investigated. Results Inhalation of CsA significantly reduced the number of eosinophils in BALF and peripheral blood, inflammatory infiltration and tissue edema of lung tissue, decreased the content of TNF-α in BALF. Conclusion Inhalation of CsA inhibited airway inflammation in rats, and the mechanism is related to inhibition of TNF-α release.

关 键 词：环孢素A 气雾吸入 气道炎症 嗜酸性粒细胞 

分 类 号：R967[医药卫生—药理学]