TRAIL诱导白血病细胞凋亡的分子机制研究  

MOLECULAR MECHANISM OF APOPTOSIS OF LEUKAMIC CELLS INDUCED BY SOLUBLE TRAIL

作　　者：祝瑾[1] 李宁川[2] 程宏[1] 

机构地区：[1]扬州大学医学院,江苏扬州225001 [2]扬州大学体育学院,江苏扬州225002

出　　处：《实用临床医药杂志》2004年第3期36-40,共5页Journal of Clinical Medicine in Practice

摘　　要：目的　检测TRAIL引起的T淋巴白血病细胞凋亡及与之相关的蛋白和信号通路 ,为探索T淋巴细胞凋亡分子机制打下基础 ,为相关肿瘤的治疗提供依据。方法　用TRAIL( 1μg/ml)刺激Jurkat细胞 3 6h以诱导细胞发生凋亡 ,用流式细胞术和MTS比色法检测细胞存活率 ,计算细胞凋亡率 ;用免疫印迹 (Westernblot)检测NF κB的表达和胱天肽酶 (caspase) 3、cas pase 8的变化。 结果　TRAIL能诱导Jurkat细胞凋亡并伴随NF κB表达增加及caspase 3和caspase 8的活化。 结论　TRAIL诱导的T淋巴白血病细胞凋亡的分子机制涉及NF κB及caspase 3、caspase 8,为TRAIL用于治疗白血病提供实验基础。S Objective: To investigate the signal pathway and expression of related proteins in apoptosis of T lymphocyte (leukemia) induced by sTRAIL, providing a novel insight and information in the apoptosis signaling pathways induced by TRAIL and an important implication for the clinic therapy of T-lymphocyte leukemia. Methods: Jurkat cells treated with TRAIL (1 μg/ml) for 36 h were used to detect the viability with MTS assay and Flow Cytometry, and to detect caspase-8, caspase-3 and NF-κB expression with Western blot. Results: TRAIL could induce apoptosis of Jurkat cells with activation of caspase-8, caspase-3 and increasement of NF-κB expression. Conclusions: Molecular mechanism of apoptosis of T-lymphocyte leukemia induced by TRAIL may involve caspase-8, caspase-3 and NF-κB may be involved in signal pathways in Jurkat cells treated by TRAIL.

关 键 词：白血病 TRAIL 细胞凋亡 NF-κB 免疫印迹 流式细胞术 

分 类 号：R392.12[医药卫生—免疫学]