雨蛙素腹腔注射法建立小鼠急性坏死性胰腺炎模型的实验研究  

Experimental Study on the Mouse Model of Acute Necrotizing Pancreatitis Induced by Intraperitoneal Injection of Caerulein

作　　者：李全生[1] 陈晓理[1] 周总光[1] 刘续宝[1] 张肇达[1] 

机构地区：[1]四川大学华西医院普外科,成都610041

出　　处：《中国普外基础与临床杂志》2004年第4期335-337,共3页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家自然科学基金(项目编号 :3 9970 93 2 )~~

摘　　要：目的　建立一种稳定的急性坏死性胰腺炎模型。方法　 36只雄性ICR小鼠用查随机数字表的方法随机分为对照组 (n =6 )和实验组 (n =30 )。实验组予以雨蛙素腹腔注射 ( 5 0 μg/kg) ,每小时注射 1次 ,共 7次 ,并分别于首次注射后 9h、18h、2 4h、4 8h和 72h各处死 6只 ;对照组予以腹腔注射等量的生理盐水 ,于注射后 18h处死。比较两组动物的血淀粉酶值、胰腺重量及胰腺组织学评分分值。结果　腹腔注射雨蛙素后 9h ,小鼠血淀粉酶值升高 ,胰腺重量增加 ,18h达峰值 ,2 4h后逐渐下降。二者与胰腺病理损害程度相一致。 18h胰腺间质炎性细胞浸润和腺泡细胞坏死明显 ,胰腺组织学评分值达到最大值 (P<0 .0 5 )。结论　大剂量雨蛙素腹腔注射能诱导ICR小鼠产生急性坏死性胰腺炎。该模型制备方法简单 ,无创伤性 ,模型稳定 。Objective To establish a mouse model of acute necrotizing pancreatitis.Methods Thirty six male ICR mice were randomly divided into control group ( n =6) and experimental group ( n =30). Each of the animals in the experimental group received 7 intraperitoneal injections of caerulein (50 μg/kg body weight) in 0.9% NaCl at hourly intervals over 6 hours. The animals in the experimental group were killed at 9,18,24,48 and 72 hours respectively after the first caerulein injection. The control animals received the same volume of 0.9% NaCl without caerulein. The animals in the control group were killed at the 18th hour after the first intraperitoneal injection. The severity of acute necrotizing pancreatitis was evaluated in terms of amylase level, pancreatic weight/body weight and the histological changes. Variance analysis was employed in the processing of these data. Results Both amylase level and pancreatic weight elevated 9 hours after the first caerulein injection, and correlated with the course of pancreatitis. The maximums of both alterations were observed at the same time point (18 hours after the first injection of caerulein). Prominent interstitial inflammation and acinar cell necrosis occurred at the 18th hour, and the histological score for pancreatitis reached a maximum ( P <0.05). Conclusion Intraperitoneal injection of a large dosage of caerulein can induce acute necrotizing pancreatitis in ICR mice. This method is simple and noninvasive, and the model established thus is stable and reproducible.

关 键 词：雨蛙素 腹腔注射 小鼠 急性坏死性胰腺炎 动物模型 实验 

分 类 号：R657.51[医药卫生—外科学] R-33[医药卫生—临床医学]