金黄色葡萄球菌L型产B型肠毒素及其基因的研究  

Study on staphylococcal enterotoxin B production and its gene of Staphylococcus aureus L-forms

作　　者：管俊昌[1] 夏佩莹[1] 唐素兰[1] 

机构地区：[1]蚌埠医学院病原生物学教研室,安徽蚌埠233003

出　　处：《蚌埠医学院学报》2004年第4期283-285,共3页Journal of Bengbu Medical College

基　　金：安徽省自然科学基金资助项目 (No .0 10 43 70 6) ;安徽省教育厅自然科学研究计划项目 (No.2 0 0 1kj172 )

摘　　要：目的 :探讨金黄色葡萄球菌 (金葡菌 )L型肠毒素B的产生量及其基因存在情况。方法 :将金葡菌诱导成稳定L型后 ,用ELISA法检测葡萄球菌肠毒素B(SEB) ,用PCR法检测SEB基因。结果 :金葡菌L型SEB的产生量与原菌比差别无显著性 (P >0 .0 5 ) ,PCR发现 4 78bp的阳性条带。结论 :金葡菌变异为L型后SEB产生量不变 ,其基因也未丢失。Objective:To investigate staphylococcal enterotoxin B(SEB) production and its gene of Staphylococcus aureus(S.aureus) L-forms.Methods:The production of SEB was examined by method ELISA and its gene was observed by PCR after S.aureus was induced into stable L-forms.Results:There was no significant difference in SEB production between S.aureus and its L-forms(P>0.05),and positive band of (478 bp) was observed by PCR in S.aureus L-forms.Conclusions:SEB production is not changed and its gene is not lost after S.aureus is changed into L-forms.

关 键 词：金黄色葡萄球菌 细菌L型 肠毒素 B型 基因 

分 类 号：R378.11[医药卫生—病原生物学]