高效液相色谱衍生化法同时测人血清中阿莫西林和克拉维酸浓度  

Simultaneous determination of serum concentration of amoxicillin and clavulanate by HPLC with fluorescence detection

作　　者：周建设[1] 申屠建中[2] 卢晓阳[2] 史美甫[2] 

机构地区：[1]杭州市萧山区第一人民医院,浙江杭州311201 [2]浙江大学医学院附属第一医院临床药理研究室,浙江杭州310003

出　　处：《中国药学杂志》2004年第7期538-539,共2页Chinese Pharmaceutical Journal

摘　　要：目的 建立同时测定人血清中阿莫西林和克拉维酸浓度的高效液相色谱法。方法 血清样品经苯甲醛衍生,荧光检测,波长λex=386 nm.λem=460 nm,采用Discovery C18柱,流动相甲醇-水=55:45,流速为1.0 mL·min-1,柱温为29℃。结果 克拉维酸和阿莫西林的保留时间分别为5.10,6.20 min左右。克拉维酸在0.062-4.968 mg·L-1内线性良好(r=0.999 9);阿莫西林在0.089-35.718 mg·L-1内线性良好(r=0.999 9)。加样回收率均在90.0％-105.0％范围内,日内和日间RSD均小于15.0％(n=5)。结论 本法灵敏度和精密度高、重复性好,适于阿莫西林克拉维酸钾复方制剂的药动学研究。OBJECTIVE: To establish a HPLC method for the simultaneous determination of amoxicillin and clavulanate in human serum. METHODS: Serum samples were pretreated with benzaldehyde, then separated on the Discovery C18 column (5 μm, 150 mm × 4.6 mm) at 29°C with MeOH-H2O = 55:45 as the mobile phase at a flow rate of 1.0 mL&middotmin-1. The fluorescence detection wavelengths were: λex = 386 nm and λem = 460 nm. RESULTS: Clavulanate and amoxicillin were eluted at about 5.10 min and 6.20 min, respectively. The calibration curves were linear (r = 0.9999) from 0.062 to 4.968 mg&middotL-1 for clavulanate and from 0.089 to 35.718 mg&middotL-1 I amoxicillin, respectively. CONCLUSION: The method is sensitive, accurate, reproducible, and suitable for the pharmacokinetic studies of amoxicillin sodium and clavulanate potassium formulations.

关 键 词：阿莫西林 克拉维酸 高效液相色谱法 

分 类 号：R917.101[医药卫生—药物分析学]