白介素2、干扰素γ、白介素10对天疱疮棘层松解影响的研究  

The Role of IL-2, IFN-γ and IL-10 in Pemphigus Acantholysis

作　　者：王鹰[1] 刁庆春[1] 钟白玉[1] 王鲁[1] 郝飞[1] 叶庆佾[1] 

机构地区：[1]第三军医大学西南医院皮肤科,重庆400038

出　　处：《中华皮肤科杂志》2004年第7期425-427,共3页Chinese Journal of Dermatology

基　　金：中华医学会皮肤科分会科研基金资助(2001042)

摘　　要：目的了解白介素2(IL-2)、干扰素γ(IFN-γ)、白介素10(IL-10)对天疱疮棘层松解的影响作用。方法借助天疱疮器官培养模型,观察不同浓度IL-2、IFN-γ、IL-10分别或共同作用24h、48h、72h后培养表皮片的棘层松解程度。结果接近正常体内浓度时,IL-2、IFN-γ对天疱疮抗体所致棘层松解无明显作用,IL-10能轻度抑制天疱疮抗体所致棘层松解;随浓度逐渐增加,IL-2、IFN-γ能显著促进天疱疮抗体所致棘层松解,IL-10则能明显抑制天疱疮抗体所致棘层松解,作用随浓度增加而更加显著;共同作用时,IL-10能抑制IFN-γ促进天疱疮抗体所致棘层松解的作用,浓度高于100pg/mL时能抑制IL-2促进天疱疮抗体所致棘层松解的作用。结论Th1型细胞因子能促进天疱疮抗体所致棘层松解,Th2型细胞因子能抑制天疱疮抗体所致棘层松解、抑制Th1型细胞因子促进天疱疮抗体所致棘层松解的作用。Objective To investigate the role of IL-2,IFN-γ and IL-10 in pemphigus acantholysis. Methods Acantholysis was observed histopathologically in the skin organ culture model of pemphigus after interacting with different concentrations of IL-2, IFN-γ and IL-10 for 24 h?48 h and 72 h. Results The acantholysis was promoted by IL-2 and IFN-γ, and the severity of acantholysis was related to the concentrations of IL-2 and IFN-γ. The effect of IFN-γ was weaker than that of IL-2. IL-10 could inhibit the acantholytic effect of IFN-γ significantly, and inhibit the acantholytic effect of IL-2 when its concentration was higher than 100 pg/mL. Conclusions Th1 cytokines can promote acantholysis induced by antibody of pemphigus (Pab) while Th2 cytokines can inhibit the acantholysis induced by Pab, and the effect of Th1 cytokines. Th2 lymphocytes may play an important role in the pathogenesis of pemphigus.

关 键 词：白介素2 干扰素Γ 白介素10 天疱疮 棘层松解 自身免疫性大疱性皮肤病 

分 类 号：R758.66[医药卫生—皮肤病学与性病学]