反义血管内皮生长因子腺病毒基因感染对类风湿关节炎滑膜细胞VEGF基因转录的影响  

Production of antisense-vascular endotheilal growth factor (VEGF) 165 adenovirus and observation of the secreatory down-regulation by the infection of the antisense-VEGF165 adenovirus

作　　者：王建华[1] 黄华杨[1] 尹庆水[1] 王捷[2] 黄盛东[3] 

机构地区：[1]广州军区广州总医院骨科,510010 [2]广州军区广州总医院医学实验科,510010 [3]上海第二军医大学胸外科实验室

出　　处：《中华风湿病学杂志》2004年第7期400-403,共4页Chinese Journal of Rheumatology

基　　金：广东省卫生厅科研基金资助项目(A2003580)

摘　　要：目的观察反义血管内皮生长因子(VEGF)165腺病毒对滑膜细胞VEGF基因转录的影响。方法将反义VEGF165腺病毒转染滑膜细胞,通过Northern-blot的方法检测其对VEGF的基因转录的影响。结果Northern杂交结果显示:反义基因转染滑膜细胞3d后,VEGF165基因的转录即受到明显抑制,4d抑制更加明显,5d后几乎检测不出阳性杂交信号。结论反义VEGF165腺病毒感染滑膜细胞后可以阻断VEGF基因的转录过程,从而抑制VEGF蛋白质的翻译和表达。Objective To observe the inhibition of vascular endotheilal growth factor (VEGF) gene transcribition and translation with antisense VEGF165 adenovirus vector transfection. Method To transfect the synovial cells with antisense VEGF165 adenovirus and detect the gene translation level with northern-blot method. Result The Northern-blot showed that: the transcription of VEGF gene were inhibited markedly after the antisense VEGF165 gene transfection for 3 days and the inhibition efficiency was obivious in 4th day. In the 5th day, the VEGF translation signal was hardly detected. Conclusion The antisense VEGF165 adenovirus gene transfection can inhibite not only the transcription but the translation of VEGF gene in synovial cells. Thus it can inhibit the secretion of VEGF in synovial cells with high efficiency.

关 键 词：反义基因 腺病毒感染 类风湿关节炎 滑膜细胞 VEGF 基因转录 血管内皮生长因子 

分 类 号：R593.22[医药卫生—内科学]