巨噬细胞炎性蛋白-1α及其受体在自身免疫性溶血性贫血患者骨髓的表达及意义  

Study on Expression and Effects of MIP-1α and CCR5 on Stem Cell of AIHA

作　　者：孙振亚[1] 张英[1] 

机构地区：[1]深圳市宝安区西乡人民医院,广东深圳518102

出　　处：《现代医院》2004年第7期4-5,共2页Modern Hospitals

摘　　要：目的　探讨骨髓巨核细胞巨噬细胞炎性蛋白 - 1α(MIP - 1α)及其受体CCR5的表达情况在自身免疫性溶血性贫血 (AIHA)的发病机制中所起的作用。方法　免疫组织化学法检测正常人、AIHA患者及其经激素治疗后骨髓片造血干细胞MIP - 1α及其受体CCR5的表达。正常人和患者骨髓造血干细胞原代培养后 ,收集上清液用ELISA测定MIP - 1α的分泌情况。结果　治疗前MIP - 1α及其受体CCR5表达明显高于治疗后及正常对照组。患者原代细胞培养上清液内MIP - 1α的含量明显高于正常对照组。结论　MIP - 1α及其受体CCR5表达的升高与AIHA的发病密切相关。Objective To study the expression and effects of MIP-1α and CCR5 on stem cell of AIHA. Methods For normal person, AIHA patients and those patients after treatment with hormone, the levels of MIP-1αand CCR5 were measured with immunohistochemistry. The stem cell of normal person and AIHA patients were cultured, then collect the upper liquid to test MIP-1α with ELISA. Results For AIHA patients MIP-1α and CCR5 were significantly higher than normal person and after treatment group. (P<0.05). In culture liquid the level of MIP-1α of patients were significantly higher than normal person and after treatment group too (P<0.05). Conclusion The rise of MIP-1α and CCR5 is closed to AIHA.

关 键 词：巨噬细胞炎性蛋白-1Α 化学激动受体5 自身免疫性溶血性贫血 免疫反应 基因表达 

分 类 号：R556.62[医药卫生—血液循环系统疾病]