高压氧对STZ大鼠糖尿病性心肌病变的治疗作用  

Role of Hyperbaric Oxygen on Treatment of Diabetic Cardiomyopathy in STZ Rats

作　　者：周亚刚[1] 刘芳[1] 邵贵强[1] 周景花[1] 管佩玲[1] 张杰[1] 钟慈声[2] 施劲东[1] 许剑锋[1] 卜国强[1] 姚庆姑[1] 

机构地区：[1]复旦大学附属上海市第五人民医院心内科,上海200240 [2]复旦大学上海医学院电镜室,上海200240

出　　处：《现代诊断与治疗》2004年第4期206-208,共3页Modern Diagnosis and Treatment

摘　　要：目的　研究高压氧治疗 (HBOT)是否可改善链脲佐菌素 (STZ)大鼠的糖尿病性心肌病变。方法　STZ大鼠造模成功 3个月、电镜证实有心肌病变后 ,随机分为三组 :( 1)HBOT组 (n =3 0 ) :每天接受 0 .15MPa、HBOT 1小时 ,连续 2 0天。( 2 )糖尿病对照组 (n =3 2 ) :不做治疗。 ( 3 )正常对照组 (n =2 0 ) :腹腔注射生理盐水的无糖尿病的正常大鼠。分别在疗程5天、10天、2 0天后处死治疗组及同期对照组大鼠 ,取心脏进行HE染色病理学观察和电镜观察。结果　HBOT 2 0天后STZ大鼠的糖尿病性心肌病变明显好于同期糖尿病对照组 ,电镜观察显示肌丝排列整齐、Z线明显、线粒体排列规则、结构紧凑。结论　HBOT可改善STZ大鼠糖尿病性心肌病变。Objective To investigate whether hyperbaric oxygen therapy(HBOT) could improve diabetic cardiomyopathy in streptozocin(STZ)-induced diabetic rats.Method Diabetes was induced in 62 SD rats by single intraperitoneal injection of 1.0% STZ.Diabetic cardiomyopathy was showed by electron micrographs.The HBOT group(n=30) was treated with HBO(0.15MPa for 1h) for 20 days and insulin.The diabetic control group(n=32) was treated with insulin only.The normal control group(n=20) undertook abdominal injection of saline.The rats in 2 groups were killed at the 5th,10th and 20th day of the course respectively,their hearts were observed with HE staining and electron microscopy.Results Diabetic cardiomyophy in treatment group was better than that in diabetic control group after 20 days of treatment of HBO.Electron micrographs of ultrastructural observation on the cardiomyocytes showed well organized myofibrils with clear Z lines and normal mitochondria.Conclusions HBOT can improve diabetic cardiomyopathy in STZ-induced diabetic rats.

关 键 词：高压氧疗法 STZ大鼠 糖尿病性心肌病变 治疗效果 DC HBOT 发病机制 治疗机理 

分 类 号：R587.2[医药卫生—内分泌]