锌对硝普钠损害精子的保护性作用研究  

Study of Zinc in Protecting Sperm from Sodium Nitroprusside Damage

作　　者：杨麦贵[1] 杨阳[2] 张竹映[3] 郝晓柯[1] 郑善銮[1] 钟立辉[1] 樊爱玲[1] 徐葳[1] 

机构地区：[1]第四军医大学西京医院检验科 [2]第四军医大学学员旅,陕西西安710033 [3]中国铁道建筑总公司西安医院小儿科,陕西西安710016

出　　处：《中华男科学杂志》2004年第7期530-532,537,共4页National Journal of Andrology

摘　　要：目的 :探讨锌对外源性一氧化氮 (NO)供体硝普钠 (SNP)损伤精子的保护性作用。　方法 :利用相差显微镜及单细胞凝胶电泳分析锌对SNP影响精子运动能力、精子头尾连接及精子DNA链断裂的保护性。　结果 :SNP对精子运动能力有明显的抑制作用 ,精子彗星细胞百分率显著增加 ,精子头尾连接稳定百分率降低 ,而锌离子能提高精子的运动功能 ,防止精子DNA链的断裂和精子头尾连接的解离。　结论 :锌对NO损伤精子有一定的保护作用 ,其机制可能与精子核染色质部位的巯基有关。Objective: To study the function of zinc in preventing human sperm from being damaged by sodium nitroprusside(SNP),an external NO donor. Methods: Analyses were made of the function of zinc in protecting sperm from being influenced by SNP in such aspects as sperm motility,head-tail connection and the breakage of sperm DNA chain by using phase-contrast microscope and single cell gel electrophoresis(SCGE). Results: Sperm motility was obviously inhibited by SNP. The percentage of comet cells increased significantly but the stability of sperm head-tail connection decreased. Zinc could promote sperm motility,protect the DNA chain and prevent the sperm head-tail connection from breaking. Conclusion: Zinc can protect sperm from being damaged by NO. Its mechanism may be related to the mercaptol group of sperm chromatin.

关 键 词：锌 精子 一氧化氮 硝普钠 DNA 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]