磷脂酶C与生殖  

Phospholipase C and Reproduction

作　　者：顾爱华[1] 周作民[1] 沙家豪[1] 

机构地区：[1]南京医科大学生殖医学实验室,江苏南京210029

出　　处：《中华男科学杂志》2004年第7期538-542,共5页National Journal of Andrology

基　　金：国家自然科学基金 ( 3 0 170 485 ) ;国家 973项目 (G19990 5 5 90 1)

摘　　要：磷脂酶C(PLC)是磷脂酰肌醇信号转导途径中一个关键酶。目前确认的哺乳类PLC共有PLC β、PLC γ、PLC δ、PLC ε和最近新发现的PLC ζ 5种亚型 ,12种同工酶。不同亚型的结构、调控和组织分布各有差异。PLC在调控信号转导通路中的特性使其在顶体反应、卵子激活中发挥了巨大的作用。本文综述了PLC家族 5种亚型的结构、调控机制及PLC在男性生殖领域的相关内容 ,包括PLC引起卵子发生Ca2 + 振荡、激活卵子、促进胚胎发育等 ,并进一步探讨了PLC在临床上可能的应用前景。Phospholipase C(PLC) is a key enzyme in phosphatidyl inositol turnover during signal transduction. The 12 mammalian PLC isozymes identified to date can be divided into five subtypes,β-type,γ-type,δ-type,ε-type and ζ-type,with extensive difference in structure,regulatory mechanism and tissue distribution. PLC plays important roles not only in sperm acrosome reaction but also in egg activation. The present studies are reviewed on the structure,regulation and function of PLC,especially its function in male reproduction,including triggering Ca 2+ oscillations in eggs to activate the eggs and helping embryo development. And the prospect of the clinical application of PLC is discussed.

关 键 词：磷脂酶C 信号转导 顶体反应 Ca^2+振荡 精子因子 

分 类 号：Q492[生物学—生理学] Q55