应用口腔粘膜细胞微核试验评价职业接触硫化氢工人的遗传损伤初探  

Preliminary Probe into the Assessment of the Genetic Damage of Occupational Hydrogen Sulfide Exposure by Micronuclei Test in Mucosa of Oral Cavity

作　　者：王玮兰[1] 朱瑞娟[1] 金复生[1] 

机构地区：[1]复旦大学金山医院,上海200540

出　　处：《工业卫生与职业病》2004年第4期205-207,共3页Industrial Health and Occupational Diseases

摘　　要：目的　探讨口腔粘膜细胞微核试验在长期接触低浓度硫化氢 (H2 S)工人的遗传损伤监护中的应用。方法　以 2 7名硫化氢作业工人为接触组 ,选择基本条件与接触组均衡而不接触硫化氢的 31名工人为对照组 ,分析其外周血淋巴细胞及口腔粘膜细胞微核率的变化。结果　外周血淋巴细胞及口腔粘膜细胞微核率有增高趋势 ,多元回归统计结果显示 ,接触硫化氢的时间长短和吸烟量与微核率呈正相关 (P <0 0 5 )。相关分析显示 ,对照组和接触组的外周血淋巴细胞微核率与口腔粘膜细胞微核率存在相关关系 (P <0 0 1 )。结论　长期接触低浓度硫化氢可能会对工人造成一定的遗传损伤。Objective To study the genetic effect of long term exposure to hydrogen sulfide by micronucleus test.Methods The micronuclei tests in peripheral blood lymphocytes and epithelial cells of mucosa in oral cavity were evaluated.Results The micronuclei on peripheral blood lymphocytes and epithelial cells of mucosa in oral cavity increased in exposure group.Linear regression was found between micronuclei in peripheral blood lymphocytes and epithelial cells of mucosa in oral cavity.Conclusions Hydrogen sulfide has some genetic damage to long term exposed workers.

关 键 词：硫化氢 微核率 外周血淋巴细胞 口腔粘膜细胞 

分 类 号：R135.1[医药卫生—劳动卫生]