自发性高血压大鼠血、尿III型前胶原水平变化及氯沙坦的干预作用  

The Effect of Lorsartan on the Serum and Urinary Levels of Procollagen Ⅲ in Sponantously Hypertensive Rats

作　　者：敖翔[1] 孙明[1] 周宏研[1] 

机构地区：[1]中南大学湘雅医院心内科,湖南长沙410008

出　　处：《中国医师杂志》2004年第8期1064-1066,共3页Journal of Chinese Physician

摘　　要：目的　探讨自发性高血压大鼠 (spontaneouslyhypertensiverat ,SHR)血、尿Ⅲ型前胶原 (ProcollagenⅢ ,PCⅢ )与早期肾脏损害的关系。方法　检测SHR及氯沙坦干预后血、尿PCⅢ水平。 14周龄雄性SHR16只随机分为高血压组和氯沙坦治疗组 (氯沙坦灌胃 3 0mg·kg-1·d-1)各 8只 ,14周龄雄性Wistar -kyoto大鼠 (WKY) 8只为正常对照组 ,16周后 ,放免法检测血、尿PCⅢ、尿微量白蛋白 (microalbumin ,ALB)及血、尿 β2 -微球蛋白 ( β2 -microglobin ,β2 -MG)。结果　SHR组尿PCⅢ、尿ALB及血、尿 β2 -MG水平明显高于WKY组 (P <0 0 5 ) ,三组血PCⅢ水平无显著差异 (P >0 0 5 )。氯沙坦可显著降低SHR组尿PCⅢ、尿 β2 -MG及尿ALB水平 ( P<0 0 5 )。血、尿PCⅢ无相关性 (r =0 2 2 6,P <0 0 5 )。尿PCⅢ与尿 β2 -MG(r =0 813 ,P <0 0 1)、尿ALB(r =0 5 60 ,P <0 0 1)、收缩压 (r =0 694,P <0 0 1)呈正相关。结论　SHR组尿PCⅢ、尿 β2 -MG、尿ALB水平升高 ,可一定程度反映高血压早期肾脏损害。氯沙坦可降低SHR血压、尿PCⅢ、尿 β2 -MG、尿ALB水平 。Objective To explore the relationships between the serum and urinary levels of procollagen Ⅲ and renal injury at the early stage of hypertension in sponantously hypertensive rats. Methods 16 sponantously hypertensive rats (SHR) were divided into treated group (losartan 30mg·kg -1·d -1) and untreated group, each group containing 8 animals, and 8 Wistar-kyoto rats served as normotensive control group(WKY). The serum and urinary levels of procollagen Ⅲ and β 2-microglobin, and urinary microalbumin level were measured by radioimmunoassay 16 weeks after treatment. Results Blood pressure, serum β 2-microglobin level, and the urinary levels of procollgen Ⅲ, β 2-microglobin and microalbumin in SHR were significantly higher than those in wistar-kyoto rats. Losartan could decease the levels of the indices above in SHR. Serum procollagen Ⅲ level had not significant difference among the three groups of rats, and was not correlated with urinary procollagen Ⅲ level. There were positive correlation between serum and urinary β 2-microglobin levels, and urinary procollgen Ⅲ level was positively retated to urinary β 2-microglobin, urinary microalbumin and systalic pressure. Conclusion The levels of urinary procollgen Ⅲ,β 2-microglobin and microalbumin could reflect the lesion of glomerulus and tubulointerstitum at the early stage of essential hypertension in SHR.

关 键 词：自发性高血压 大鼠 血 尿 Ⅲ型前胶原 氯沙坦 干预作用 SHR 心肌纤维 

分 类 号：R544.1[医药卫生—心血管疾病]