肺心合剂对肺动脉高压大鼠肺血管重建的影响  

Infulence of Feixin mixture(肺心合剂) on remodelling of monocrotaline induced pulmonary arterial structure in rats

作　　者：杨茂农[1] 胥方元[2] 王毅[3] 王飞[3] 刘跃建[1] 朱春冬[3] 严晓梁[1] 吉德平[1] 

机构地区：[1]四川省人民医院,四川成都610072 [2]泸州医学院附属医院,四川泸州646000 [3]成都中医药大学临床医学院,四川成都610072

出　　处：《中国中西医结合急救杂志》2004年第4期231-234,共4页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：四川省中医药管理局科研基金资助项目(96031)

摘　　要：目的 :探讨肺心合剂对野百合碱诱发肺动脉高压大鼠肺血管重建的影响。方法 :利用野百合碱(5 0 mg/ kg)复制大鼠肺心病模型。 6 0只雄性 Wistar大鼠随机分为正常对照组 ,模型组 ,肺心合剂低、中、高剂量组及硝苯地平组 6组 ,每组 10只。分别于制模后 14 d灌胃相应药物 ,连续用药 8d。处死动物后 ,利用特殊染色结合病理图像分析方法测定肺小动脉病理 (光镜下观察和弹力纤维染色切片 )及其形态计量学 (指标为与终末细支气管伴行的肺小动脉管壁中膜厚度、外径、管壁面积、室管腔面积以及与血管外径的比值 )。结果 :肺心合剂及硝苯地平均能明显减轻模型大鼠的肺血管重构 (P均 <0 .0 1) ,以肺心合剂高剂量组效果最好 ;但治疗组各指标仍未完全恢复到正常对照组水平。结论 :肺心合剂能部分逆转肺血管结构重建 ,有效降低肺动脉高压。Objective: To explore the reversal effect of Feixin mixture(FXM, 肺心合剂) on remodelling of monocrotaline(MCT) induced pulmonary arterial structure in rats. Methods: Adult male Wistar rats were given a single dose of MCT(50 mg/kg) to induce the model of pulmonary hypertension. Sixty rats were (randomly) divided into normal control group, model group, FXM group of low, middle, large dosage and (Nifedipine) group(n=10 in each group). Then the rats were treated with gastric infusion of normal saline, (different) dosage of FXM or Nifedipine respectively. The small pulmonary arterial morphologic changes of rats lung were measured by optical microscope with hematoxylin and eosin(HE) and elastic fiber stains combined with image analysis, including middle membrane thickness, external diameter, wall area, cavity area, as well as the ratio of the middle membrane thickness and external diameter of the small pulmonary artery, which companied with the terminal bronchiole. Results: FXM and Nifedipine could significantly inhibit the structural remodelling of small pulmonary arterial induced by MCT(all P<0.01). The effects of high dosage FXM (group) were better. But the indexes were not recovered to the normal in each treatment group. Conclusion: FXM is able to partly reverse the change in structure of pulmonary arterial and reduce the pulmonary (hypertension).

关 键 词：肺心合剂 野百合碱 肺动脉高压 肺血管重建 

分 类 号：R285[医药卫生—中药学]