补肾益气方对正常早孕期人细胞滋养层细胞生物学行为的影响  

Effect of Bushen Yiqi Recipe on Bioactivity Behavior of Human Cytotrophoblast of Early Pregnancy

作　　者：王海燕[1] 归绥琪[1] 徐丛剑[1] 储以微[2] 

机构地区：[1]复旦大学附属妇产科医院中西医结合科,上海200011 [2]复旦大学上海医学院免疫教研室

出　　处：《中国中西医结合杂志》2004年第6期525-528,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：上海市科学技术发展基金资助项目(No .98- 2 0 3);上海市医学领先学科中西医结合妇科重点学科资助项目;复旦大学 985工程资助项目

摘　　要：目的探讨补肾益气方对正常早孕期人细胞滋养层细胞增殖、侵袭、分化等生物学行为的影响。方法体外培养正常早孕期人细胞滋养层细胞 ,分别设空白对照组、5 %含药血清组、10 %含药血清组、2 0 %含药血清组 ,用扫描电镜、噻唑蓝 (MTT)法、流式细胞仪、Transwell侵袭实验检测含药血清作用 2 4h、4 8h、72h后细胞的形态、增殖活性及侵袭力的变化。结果含药血清培养 2 4h、4 8h、72h后 ,细胞滋养层细胞微绒毛变丰富 ,伪足增多、延长 ;细胞在酶联免疫分析仪 4 90nm波长的吸光度值增加 (P <0 0 1) ;sub G1期细胞减少 (P <0 0 1) ,S期细胞增加 (P <0 0 1) ,G2 /M期细胞明显减少 (P <0 0 1) ;每视野穿过PET膜的细胞数明显增多 (P <0 0 1)。结论补肾中药促进人细胞滋养层细胞增殖及侵袭力 。ObjectiveTo investigate the effect of Chinese drugs for tonifying Shen on the bioactivity, cell proliferation, invasion and differentiation of human cytotrophoblast of early pregnancy. MethodsThe human early pregnant cytotrophoblast was cultured in vitro, and treated with saline (as blank control) and drug containing serum in different concentrations (5%, 10% and 20%) respectively. The changes of morphology, proliferation and invasive capacity of cells were examined by scanning electron microscopy, MTT method, flow cytometry, Transwell invassive assay at 24 hrs, 48 hrs and 72 hrs after treatment. ResultsAfter cells being cultured with drug containing serum, the cytotrophoblast became abundant in microvilli, with more and prolonged pseudopodia. The cell absorbency in 490nm wave length increased significantly (P<0 01), cells of sub G 1 and G 2/M phase obviously decreased and that of S phase increased (P<0 01), and the cells penetrated through PET membrane in each visual field significantly increased (P<0 01). ConclusionChinese herbs for tonifying Shen could promote the proliferation and invasive capacity of cytotrophoblast and might influence its differentiation.

关 键 词：补肾益气方 早孕期 细胞滋养层 细胞生物学行为 胎盘功能 

分 类 号：R285[医药卫生—中药学]