端粒酶活性及hTERT mRNA表达在鉴别肝脏良恶性病变中的意义  

Clinical Significance of Telomerase Activity and Human Telomerase Reverse Transcriptase mRNA Expression for Differential Diagnosis of Malignant and Benign Liver Lesions

作　　者：范瑞芳[1] 卫立辛[1] 吴孟超[1] 沈锋[1] 王维锋[1] 贾凤岐[1] 卜欣欣[1] 郭献灵[1] 尤天庚[1] 杨家和[1] 郭亚军[1] 

机构地区：[1]第二军医大学东方肝胆外科医院肿瘤免疫和基因治疗中心,上海市200438

出　　处：《中国肿瘤临床》2004年第14期781-783,787,共4页Chinese Journal of Clinical Oncology

基　　金：国家重点基础研究发展规划(973项目)基金资助(编号:G2000057001)

摘　　要：目的:研究端粒酶活性及人端粒酶逆转录酶(hTERT)mRNA表达在鉴别肝脏良、恶性病变中的意义。方法:采用ELISA-TRAP法检测了130份外科切除肝脏样本及58个肝脏可疑结节的端粒酶活性,RT-PCR法检测hTERTmRNA表达。所有样本均经病理学证实。结果:外科切除的肝细胞癌、肝硬变及慢性肝炎组织端粒酶阳性率分别为85.9%(55/64)、25.0%(8/32)及8.3%(2/24),hTERTmRNA阳性率分别为89.1%(57/64)、25.0%(8/32)及8.3%(2/24),10份正常肝脏组织端粒酶及hTERTmRNA表达阴性。经皮肝穿刺活检的肝细胞癌、胆管细胞癌、局灶性结节性增生、炎性假瘤及腺瘤样增生组织的端粒酶阳性率分别为85.7%(30/35)、100%(4/4)、33.3%(4/12)、25.0%(1/4)及33.3%(1/3),hTERTmRNA阳性率分别为88.6%(31/35)、100%(4/4)、33.3%(4/12)、25.0%(1/4)及33.3%(1/3)。肝脏恶性肿瘤端粒酶及hTERTmRNA阳性率明显高于肝脏良性病变(P<0.01)。结论:经皮肝穿刺活检端粒酶活性及hTERTmRNA检测有助于肝脏良、恶性病变的鉴别诊断。Objective: To study the clinical significance of telomerase activity and human telomerase reverse transcriptase (hTERT) mRNA expression for differential diagnosis of malignant and benign liver lesions. Methods: Telomerase activity was detected by ELISA-TRAP assay in 130 surgical resected liver samples and 58 percutaneous biopsied liver samples. Expression of hTERT mRNA was also measured by RT-PCR assay in these samples. Postoperative pathological examination was performed for every case. Results: Among the 130 surgical liver samples, the positive rates of telomerase activity in hepatocellular carcinoma (HCC), liver cirrhosis, chronic hepatitis tissues were 85.9%(55/64), 25.0%(8/32) and 8.3%(2/24) respectively, and the positive rates of hTERT mRNA expression were 89.1%(57/64), 25.0%(8/32) and 8.3%(2/24) respectively. Neither telomerase activity nor hTERT mRNA expression was detected in 10 normal liver tissues. Among the 58 biopsied liver specimens, the positive rates of telomerase activity in HCC, cholangiocellular carcinoma, focal nodular hyperplasia, inflammatory pseudotumor and adenomatous hyperplasia tissues were 85.7%(30/35), 100%(4/4), 33.3%(4/12), 25.0%(1/4) and 33.3%(1/3) respectively, and the positive rates of hTERT mRNA expression were 88.6%(31/35), 100%(4/4), 33.3%(4/12), 25.0%(1/4) and 33.3%(1/3) respectively. The positivity of telomerase activity or hTERT mRNA expression in malignant liver tumors was significantly higher than that of in benign liver lesions (P<0.01). Conclusion: Detection of telomerase activity or hTERT mRNA expression in percutaneous biopsied liver tissues may be useful for differential diagnosis of malignant and benign liver lesions.

关 键 词：肝脏肿瘤 端粒酶 人端粒酶逆转录酶 鉴别诊断 

分 类 号：R446.6[医药卫生—诊断学]