替硝唑对大鼠血胰屏障通透性的研究  

Transference of Tinidazole across the Blood-pancreas Barrier in Rats

作　　者：李功华[1] 祁金文[1] 马珂[1] 

机构地区：[1]浙江省人民医院药剂科,杭州310014

出　　处：《医药导报》2004年第8期525-527,共3页Herald of Medicine

摘　　要：目的 :研究替硝唑对大鼠血胰屏障的通透性 ,为选择合适的抗微生物药物治疗继发性胰腺组织感染提供理论依据。方法 :给予大鼠替硝唑 2 0mg·kg 1 ,sc ,在规定时间点取样 ,用高效液相色谱法 (HPLC)测定胰腺组织和血清中药物的浓度。结果 :血清、胰腺组织中替硝唑浓度在 2 0min时最高 ,分别为 19.73 μg·mL 1 ,5 5 .17μg·g 1 ;药物对血胰屏障的通透率 (PR) >1。结论 :皮下注射替硝唑后 ,药物在胰腺中有较高的分布 ,对血胰屏障具有良好的通透性 ,值得向临床推荐用于预防和治疗胰腺感染。Objective:To probe into the transference of tinidazole across the blood-pancreas barrier in rats so as to provide theoretical basis for the selection of appropriate antimicrobics for the treatment of secondary infections of the pancreas. Methods:35 SD rats served as the subjects of the study. Each of the animals was given a subcutaneous injection of tinidazole (20 mg·kg -1 body weight). Blood samples were taken 0,5,10,20,30,60,90,120,180 and 240 min separately after the injection and sera were separated. Pancreata of the animals were taken out separately at the same time points as mentioned above for the preparation of tissue homogenates. The concentrations of tinidazole in the sera and pancreatic tissue homogenates were determined with high performance liquid chromatography (HPLC). Results:The concentrations of tinidazole in sera and pancreatic tissue homogenates reached their peak values of 19.73 μg·mL -1 and 55.17 μg·g -1 20 min after the injection,respectively. The permeation ratio of the drug through the blood pancreas barrier was greater than 1. Conclusion:After a subcutaneous injection of tinidazole in the rat the drug was shown to have a fairly high distribution in the pancreas,denoting its good tranferability across the blood-pancreas barrier. It is therefore proposed by the authors that tinidazole may be used clinically for the prevention and treatment of pancreatic infections.

关 键 词：替硝唑 血胰屏障 通透率 

分 类 号：R978.69[医药卫生—药品] R576[医药卫生—药学]