去脂胶囊质量标准的研究  

Studies on quality standard for Quzhi Capsules

作　　者：彭荣珍[1] 伍毅[1] 

机构地区：[1]暨南大学医学院第六附属医院

出　　处：《中成药》2004年第7期544-546,共3页Chinese Traditional Patent Medicine

摘　　要：目的 :建立了去脂胶囊 (大黄 ,决明子 ,茵陈 ,苍术等 )的质量控制方法。方法 :采用薄层色谱法对去脂胶囊中的茵陈、苍术进行定性鉴别 ;并采用HPLC法对该药中的大黄酚进行含量测定 ,色谱条件 :用Shim packVP ODS(4 .6mm×2 5 0mm)柱 ,以甲醇 0 .5 %磷酸 (85∶1 5 )为流动相 ,检测波长为 2 5 4nm ,进样量为 2 0 μL。 结果 :薄层色谱图斑点清晰 ,阴性对照无干扰 ;高效液相色谱法测定大黄酚在 1 .8～ 1 4 .4 μg·mL-1 范围内呈良好的线性关系 (r =0 .9999)。平均回收率为1 0 1 .0 % ,RSD =1 .3%。结论 :该方法准确、灵敏。AIM: To establish the method for quality control of Quzhi Capsules(Radix et Rhizoma Rhei, Semen Cassiae, Herba Artemisiae Scopariae, Rhizoma Atractylodis, etc.). METHODS: Herba Artemisiae and Rhizoma Atractylodis in this medicine were identified by TLC. The content of chrysophanol was determined by HPLC on ODS column. The mobile phase was composed of methanol 0.5% phosphoric acid(85∶15,v∶v) and detection wavelength was at 254nm. RESULTS: The spots on TLC plates were clear without interference in the blank reference. The linearity of HPLC was good( r =0.9999), and the average recovery was 101.00%, RSD =1.3%. CONCLUSION: This method is simple, sensitive and accurate, and available for control of Quzhi Capsules.

关 键 词：去脂胶囊 茵陈 苍术 大黄酚 TLC HPLC 

分 类 号：R927.2[医药卫生—药学]