银杏叶提取物对糖尿病大鼠睾丸损伤的作用  

Protective effect of Ginkgo biloba extract on testicular tissue of diabetic rats

作　　者：毛孙忠[1] 陈国荣[2] 雷康福[1] 李旭升[2] 王芳[2] 李剑敏[2] 方周溪[3] 胡芸[2] 

机构地区：[1]温州医学院生物化学教研室,浙江温州325027 [2]温州医学院附属第一医院病理科,浙江温州325027 [3]温州医学院电镜室,浙江温州325027

出　　处：《中国病理生理杂志》2004年第7期1238-1242,共5页Chinese Journal of Pathophysiology

基　　金：温州市科技发展计划项目 (No.Y2 0 0 3A0 4 2 ) ;浙江省教育厅项目 (No .2 0 0 0 0 6 74 )

摘　　要：目的 :研究银杏叶提取物 (GBE)对糖尿病所致的睾丸损伤的作用及其机制。方法 :用光镜及透射电镜观察链脲佐菌素 (STZ)诱导的糖尿病 1个月大鼠睾丸的形态学改变 ,并测定睾丸组织丙二醛 (MDA)、NO产物NO2 -/NO3 -的含量及超氧化物歧化酶 (SOD)、一氧化氮合酶 (NOS)的活性。结果 :①糖尿病大鼠睾丸组织光镜下主要表现为睾丸曲细精管萎缩、变形及生精上皮脱落 ,透射电镜下主要见到支持细胞浆内内质网扩张、脂滴空泡形成 ,溶酶体明显减少 ,银杏叶组大鼠睾丸组织光镜、透射电镜下上述病变明显改善。②睾丸组织的MDA、NO2 -/NO3 -含量及tNOS和iNOS活性银杏叶组低于糖尿病组 ,SOD活性银杏叶组高于糖尿病组。结论 :GBE对糖尿病所致的睾丸损伤有明显的保护作用 ,其作用机制与提高SOD活力减少MDA产生 。AIM: To investigate the protective effect of Ginkgo biloba extract (GBE) on diabetic testis and explore its possible mechanism. METHODS: Testicular structure of streptozotocin-induced diabetic rats was observed under light microscopy (LM) and transmission electron microscopy (TEM). Content of malondialdehyde (MDA), NO_2^-/NO_3^- and activity of superoxide dismutase (SOD), nitric oxide synthase (NOS) were determined in testicular homogenate. RESULTS: In diabetic rats, it was manifestated as deformation of seminiferous tubule, atrophy and shedding of germinal epithelium under LM, while expansion of smooth endoplasmic reticulum, formation of fatty vacuoles and decrease of lysosome obviously in the cytoplasm of sertoli cell under TEM, the injury of testicular tissue was improved by GBE. Compared with diabetic rats, activity of SOD increased while activity of tNOS and iNOS, content of MDA and NO_2^-/NO_3^- decreased in GBE-treated rats. CONCLUSION: GBE could effectively prevent the development of diabetic testis and the effect may be partly achieved by resisting lipid peroxidation,restraining the activity of testicular tissue iNOS and reducing the pathological alterations of NO. [

关 键 词：糖尿病 实验性 睾丸 脂质过氧化作用 一氧化氮 二裂银杏 

分 类 号：R587.1[医药卫生—内分泌]