从噬菌体构象型7肽库中筛选BLyS的抑制性短肽  

Screening of antagonist peptide of BLyS from C7C phage display peptide library

作　　者：吉清[1] 何凤田[1] 李蓉芬[1] 高会广[1] 郑英如[2] 钟小林[1] 郑汉其[3] 

机构地区：[1]第三军医大学生物化学与分子生物学教研室,重庆400038 [2]第三军医大学大坪医院妇产科,重庆400042 [3]香港中文大学生物化学系

出　　处：《细胞与分子免疫学杂志》2004年第4期398-401,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目 (No .30 2 71 2 2 8;30 370 31 9)

摘　　要：目的 :从噬菌体构象型 7肽库中筛选BLyS的抑制性短肽。方法 :用重组人BLyS筛选噬菌体构象型 7肽库 ,用间接ELISA、竞争ELISA和细胞增殖活性试验鉴定噬菌体克隆。结果 :经过 3轮亲和筛选 ,噬菌体的回收率增加 ,阳性克隆得到富集。所获两个阳性噬菌体克隆能特异性地抑制BLyS刺激细胞增殖的活性。结论 :获得了噬菌体呈现的能够抑制BLyS的两个短肽。AIM: To screen an antagonist peptide of BLyS from C7C phage display peptide library. METHODS: C7C phage display peptide library was screened with BLyS. Indirect ELISA, competitive ELISA and MTT colorimetry were used to identify positive phage clones. RESULTS: After 3 rounds of sceening, the gradual increase of the ratio of output to input and specific enrichment had been achieved. Two phage clones that could inhibit the BLyS activity were identified. CONCLUSION: Two phage display antagonist peptides of BLyS were obtained.

关 键 词：BLYS 噬菌体肽库 小肽 

分 类 号：Q81[生物学—生物工程]