IL-18和HIV-1gag-gp120嵌合基因DNA疫苗联合免疫小鼠的免疫应答  

The immune response in mice inoculated with HIV-1 gag-gp120 chimeric gene DNA vaccine and IL-18 plasmid

作　　者：江文正[1] 金宁一[1] 李子健[2] 张立树[1] 王宏[1] 金洪涛[1] 

机构地区：[1]解放军军需大学全军基因工程重点实验室,吉林长春130062 [2]吉林大学第一医院,吉林长春130023

出　　处：《细胞与分子免疫学杂志》2004年第4期433-435,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家杰出青年科学基金资助 (No .3982 51 1 9);国家高技术研究发展计划 (863)资助项目 (No.2 0 0 1AA2 1 50 31 )

摘　　要：目的 :探讨IL 18和HIV 1gag gp12 0嵌合基因的DNA疫苗联合免疫小鼠的免疫应答。方法 :构建含IL 18的真核表达质粒pVAXIL18,将他与表达HIV 1gag gp12 0嵌合基因的核酸疫苗质粒pVAXGE共同肌注免疫BALB/c小鼠 ,检测免疫小鼠脾特异性CTL杀伤活性和血清抗体滴度。结果 :联合免疫组小鼠脾特异性CTL杀伤活性和血清抗体水平均显著高于单独免疫组 (P <0 .0 5 ) ,空白质粒对照组 (P <0 .0 1)和PBS对照组 (P <0 .0 1)。结论 :IL 18和HIV 1gag gp12 0嵌合基因的DNA疫苗联合免疫可诱导小鼠产生特异性细胞和体液免疫 ,且IL 18发挥了免疫佐剂的作用。AIM: To explore the immune response in mice inoculated with HIV-1 chimeric gene gag-gp120 DNA vaccine and IL-18 plasmid. METHODS: BALB/c mice were injected i.m with eukaryotic expression plasmid pVAXIL18 containing IL-18 gene and DNA vaccine plasmid pVAXGE containing HIV-1 gag-gp120 chimeric gene. The specific killing activities of spleen CTL and the titers of serum antibodies of the immunized mice were detected. RESULTS: The specific killing activities of spleen CTL and the titers of serum antibodies in the co-inoculation group were significantly higher than those in the single inoculation group(P<0.05), the vector control group (P<0.01) and PBS control group (P<0.01). CONCLUSION: The specific cellular and humoral immunity in mice can be induced by co-inoculating HIV-1 gag-gp120 chimeric gene DNA vaccine and IL-18 plasmid indicating that IL-18 augments immune response as immunoadjuvant.

关 键 词：HIV-1 gag-gp120 IL-18 DNA疫苗 

分 类 号：R373.9[医药卫生—病原生物学]