转基因小鼠高表达的FasL对Sertoli细胞免疫调节功能的影响  

Influence of FasL overexpression in transgenic mice on the immune regulative function of sertoli cell

作　　者：刘照平[1] 李伟毅[1] 林波[2] 马映华[2] 胡佳华[2] 陈广洁[1] 席晔斌[1] 王保国[1] 包士三[3] 郭礼和[2] 

机构地区：[1]上海市第二医科大学上海市免疫学研究所,上海200025 [2]中国科学院上海生物化学与细胞生物学研究所,上海200031 [3]澳大利亚悉尼大学病理系

出　　处：《细胞与分子免疫学杂志》2004年第4期456-460,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目 (No .39970 2 83)

摘　　要：目的 :研究转基因小鼠高表达的FasL对Sertoli细胞在睾丸局部感染时的免疫调节作用的影响。方法 :将溶脲脲原体 (UU)分别注入FasL转基因及野生型小鼠膀胱 ,模拟上行性感染的途径。分别在感染后 1、2和 3wk处死小鼠 ,分离睾丸组织观察其病理变化 ,并用免疫组化染色法比较UU感染前后 ,Ser toli细胞上FasL和TGF β、IL 1α、IL 6的表达及分泌格局的差异。从野生型小鼠睾丸组织中分离高纯度的Sertoli细胞 ,与未感染UU的对照组相比 ,观察UU感染后FasL+ Sertoli细胞介导Fas+ Jurkat细胞的凋亡能力的变化。结果 :UU感染组的转基因小鼠 ,睾丸组织发生的病理改变比野生型更为明显 ;两种小鼠感染后Sertoli细胞分泌的调节因子变化格局不同 ;感染后Sertoli细胞对Jurkat细胞的杀伤能力增强。结论 :在抗感染免疫中 ,转基因表达的FasL可影响Sertoli细胞分泌细胞因子的格局 ,进而影响睾丸局部的免疫平衡。过高表达的FasL对机体的抗感染应答并非一定有利。AIM: To study the influence of FasL overexpression in transgenic mice on the Sertoli cell immune response to testicular infection. METHODS: Ureaplasma urealyticum (UU) was directly injected into bladders of FasL transgenic mice and wild-type mice respectively, to mimick an ascending infection pathway. At week 1, 2 and 3 after injection, respectively, the mice were put to death to observe the pathological alterations in testis section. And at the same time the differences of FasL, TGF-β, IL-1α and IL-6 expressions on Sertoli cells were compared by immunohistochemical staining between wild mice and transgenic mice before and after infection, respectively. The high-purified Sertoli cells were isolated from the testis tissue of wild-type mice, comparing apoptotic capability of Fas+ Jurkat cells mediated by FasL+ Sertoli cells of wild control and wild UU-infected groups. RESULTS: The pathological changes of testis tissue from transgenic mice were more serious as compared with wild-type mice and the model of cytokines secreted by sertoli cells was distinctive between the two kinds of mice. The UU-infected Sertoli cells increased Fas+ Jurkat cell apoptosis. CONCLUSION: FasL overexpression can influence the cytokine’s secretion in the process of anti-infection immunity and further affects the immune balance of testis locality. Not all FasL over-expression is benefit to body’s anti-infection immune response.

关 键 词：FASL 转基因小鼠 SERTOLI细胞 免疫调节 

分 类 号：R392.12[医药卫生—免疫学] R392.23[医药卫生—基础医学]