高效液相色谱法测定抗敏Ⅲ号袋泡茶中丹参素的含量  

Determination of Danshensu in Kangming Ⅲ Teabag by HPLC

作　　者：许根英[1] 杨春欣[1] 董颖[1] 吕迁洲[1] 

机构地区：[1]复旦大学附属中山医院药剂科,上海200032

出　　处：《复旦学报（医学版）》2004年第4期432-433,共2页Fudan University Journal of Medical Sciences

摘　　要：目的　建立高效液相法测定抗敏Ⅲ号袋泡茶中丹参素含量的方法。方法　固定相为Diamonsil(TM ) ,C18,2 5 0× 4 .6mm ,5 μm ,流动相为 0 .5 %HAc水溶液∶甲醇 ( 90∶10 ) ,流速为 1mL/min ,检测波长为 2 81nm ,柱温35℃ ,进样量 2 0 μL。结果　丹参素在 0 .6 2 2～ 6 .2 2 μg呈良好的线形关系 (r =0 .9999) ,平均加样回收率为99.7% ,RSD =1.13% (n =6 )。结论　用本法测得三批袋泡茶中丹参素含量分别是 5 .71、5 .16、5 .4 6mg/袋 ,本方法简便、准确 ,重现性好 ,可用于抗敏Ⅲ号袋泡茶的质量控制。Purpose To establish a RP HPLC assay for the determination of Danshensu in Kangming Ⅲ teabag. Methods The chromatographic column was Diamonsil (TM),C 18 ,250×4.6 mm,5 μm.A mixture of methanoL 0.5% acetic acid (10∶90) was used as the mobile phase with a flow rate of 1 mL/min and the detection wavelength was 281 nm at 35?℃. Results The calibration curve for Danshensu showed good linear correlation within the concentration range of 0.622～6.22 μg( r=0.999 9 ).The average recovery and RSD was 99.7% and 1.13% ( n=6 ) respectively. Conclusions The content of Danshensu was 5.71 、5.16、5.46 mg in Kangming Ⅲ teabag separately.The method is convenient,reliable and with good reappearance and can be used for the quality controL of Kangming Ⅲ teabag.

关 键 词：高效液相色谱法 测定 抗敏Ⅲ号袋泡茶 丹参素 含量 

分 类 号：R927.2[医药卫生—药学]