ABC-ELISA检测血清粉尘螨特异性IgE  

Detection of mite-specific IgE in sera of asthmatics by ABC-ELISA

作　　者：崔玉宝[1] 

机构地区：[1]安徽理工大学医学院病原生物学教研室,安徽232001

出　　处：《中国人兽共患病杂志》2004年第7期617-618,623,共3页Chinese Journal of Zoonoses

摘　　要：目的　应用ABC -ELISA法检测粉尘螨特异性IgE。 方法　按常规ABC -ELISA法操作 ,以阳性血清光密度值1 0时所需要的条件为最适条件 ,经预实验选择最适抗原包被浓度、血清稀释度和酶结合物浓度 ,并经特异性抑制试验验证。用ABC -ELISA法对 76例哮喘患者和 30例正常人进行粉尘螨特异性IgE检测 ,并与皮肤挑刺试验结果比较。 结果　ABC -ELISA法检测粉尘螨特异性IgE最适抗原包被浓度为 10 μg/ml,血清稀释倍数为 1∶4 0 ,Avidin -HRP为 1∶6 0 0 ,粉尘螨浸液和鼠抗人IgE均能抑制阳性反应。支气管哮喘患者和正常人粉尘螨特异性IgE校正OD值分别为 2 4 1± 1 13和 1 2 5± 0 5 6 ,差异具显著性 (χ2 =5 36 ,P <0 0 1)。粉尘螨浸液皮试与螨特异性IgE测定的符合率为 93 4 0 % (99/10 6 )。 结论　ABC -ELISA法检测粉尘螨特异性IgE耗时短 ,且具有较好的抗原特异性 ,建议临床推广应用。The ABC-ELISA assay was used to detect the mite-specific IgE in sera of 76 asthmarics and 30 healthy individuals This method of testing was performed on the proper conditions:when the optic density (OD) value of positive sera was 1.0.,the best concentration of coating antigen,serum dilutions and concentration of enzyme-conjugates were pre-tested before use and verified by specific inhibition test.The results were compared with those of skin prick test (SPT).It was found the best concentration of coating antigen was 10μg/ml.; the proper dilution of serum was 1∶40 and the concentration of avidin-HRP was 1∶600.Both the extract of Dermatophagoides farine and the mouse anti-human IgE antibody could suppress the positive reactions and the corrected OD values of the asthmatics and healthy individuals were 2.41±1.13 and 1.25±0.56 with significant differences (χ2=5.36,P<0.01).The coincidence rate between the results of the SPT with P.farine extract and those with ABC-ELISA was 93.4% (99/106) It concludes that ABC-ELISA is a method of choice for the detection of the mite-specific IgE in sera of asthmatics,because of its short time of performance and good antigenic specificity.

关 键 词：粉尘螨特异性IgE 皮肤挑刺试验 ABC^ELISA法 哮喘 

分 类 号：R384.4[医药卫生—医学寄生虫学]