停流-荧光反应速率仪及其应用于测定维生素B_1  

Stopped-flow Fluorescence Reaction Rate Analyzer and Its Application for the Determination of Thiamine

作　　者：杨屹[1] 蔡汝秀[1] 黄厚评[1] 曾云鹗[1] 

机构地区：[1]武汉大学化学系,武汉430072

出　　处：《分析化学》1993年第3期360-363,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金

摘　　要：本文利用国产930型荧光光度计作为检测器,采用自已制作的流通池,配以蠕动泵及记录仪,组装成停流-荧光动力学分析装置,并用以进行了停流荧光反应速率法测定维生素B_1的研究,利用Hg^(2+)离子在碱性介质中将硫胺素(维生素B_1)氧化为硫胺荧的动力学反应,测定了维生素B_1片剂中维生素B_1的含量。结果表明,这套仪器用于动力学分析,操作简便、省时,维生素B_1的测定结果与标准值基本相符,相对标准偏差为1.28％,其准确度和重现性均令人满意。A stopped-flow fluorescence reaction rate analyzer was developed. It consisted of a fluorescence photometer (Model 930) with a self-made flow-through cell, a peristaltic pump and a recorder. The reaction involving the oxidation of thiamine by Hg2- in basic solutions to fluorescent thiochrome was studied. The content of thiamine in vitamin B1 tablet was determined with satisfactory results(RSD<l. 3%).

关 键 词：荧光反应速率 维生素B1 停流技术 

分 类 号：Q563.1[生物学—生物化学]