利血平的光化学荧光特性及其应用  

Photochemical-Fluorescence Nature of Reserpine and Its Application

作　　者：欧阳耀国[1] 蔡维平[1] 林荣辉 许金钩[1] 

机构地区：[1]厦门大学化学系,厦门361005

出　　处：《分析化学》1993年第4期422-424,共3页Chinese Journal of Analytical Chemistry

摘　　要：在10％HAc介质中,利血平经紫外光照射时,其光化学反应产物具有强烈荧光(λ_(ax)=385 nm,λ_(ax)=490 nm)。利血平浓度在0.01～0.30μg/ml范围内,荧光强度与浓度呈良好的线性关系,由此建立了利血平的光化学荧光测定法。方法的检出限为1.3 ng/ml,相对标准偏差为1.6％。本法简便快速且灵敏度高,可用于片剂及注射液中利血平含量的测定。In the medium of 10% acetic acid, reserpine leads to a fluorescent photochemical reaction product under the irradiation of ultraviolet light. The excitation and emission wavelengths of the product are 385 and 490 nm, respectively. On this basis, a photochemical-fluorimetric method for the determination of reserpine was developed with a detection limit of 1. 3 ng/ml and a relative standard deviation of 1. 6%. The linear relationship between the fluorescence intensity and the concentration of reserpine is over the range of 0. 01-0. 30 μg/ml. This method is simple, rapid and sensitive, and has been used for the determination of reserpine in the tablets and injections.

关 键 词：利血平 光化学法 荧光分析法 

分 类 号：R972.4[医药卫生—药品]