新荧光试剂5-(4′-氯-2′-羧基苯基偶氮)-4-氧代-2-硫代四氢噻唑的合成及分析应用研究  

Synthesis of a New Fluorescent Reagent 5- (4'-Chloro -2'-carboxylphenylazo)-rhodanine and Its Application

作　　者：史好新[1] 王忠义[1] 史海健[1] 杨周生[2] 郑爱华[2] 

机构地区：[1]安徽师范大学化学系,芜湖241000 [2]安徽师范大学测试中心

出　　处：《分析化学》1993年第4期461-463,共3页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金

摘　　要：本文报道了新荧光试剂5-(4’-氯-2’-羧基苯基偶氮)-4-氧代-2-硫代四氢噻唑(CC-POST)的合成及其荧光分析应用。确证了其结构。在pH=5.0～5.4时。它与铜(Ⅱ)形成荧光螯合物。λ_(ex)/λ_(em)=310mn/408nm,检测限为0.5ng/g。用此法测定了茶叶中的痕量铜,结果满意。The synthesis and fluorimetric analysis of 5-(4'-chloro-2'-carboxylphenylazo)-rhodanine (CCPOST) are studied. The product has been characterized by elemental analysis, ER, NMR and MS. CCPOST reacts with copper( Ⅱ ) to form a stable complex at pH 5. 0-5- 4,Which shows an intense fluorescence at λex/ λem= 310 nm/408 nm. The molar ratio of Cu( Ⅱ ) : CCPOST in the complex is estimated to be 1 : 1. The detection limit of CCPOST is 0. 5 ng/g. The method has been applied to the determination of trace copper in tea with satisfactory results.

关 键 词：荧光分析 特效试剂 CC-POST 

分 类 号：O657.3[理学—分析化学]